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Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines

, : Effect of lycopene on cell viability and cell cycle progression in human cancer cell lines. Cancer Cell International 12(1): 36-36

Lycopene, a major carotenoid component of tomato, has a potential anticancer activity in many types of cancer. Epidemiological and clinical trials rarely provide evidence for mechanisms of the compound's action, and studies on its effect on cancer of different cell origins are now being done. The aim of the present study was to determine the effect of lycopene on cell cycle and cell viability in eight human cancer cell lines. Human cell lines were treated with lycopene (1-5 uM) for 48 and 96 h. Cell viability was monitored using the method of M. The cell cycle was analyzed by flow cytometry, and apoptotic cells were identified by terminal deoxynucleotidyl transferase-mediated dUTP nick labeling (TUNEL) and by DAPI. Our data showed a significant decrease in the number of viable cells in three cancer cells lines (HT-29, T84 and MCF-7) after 48 h treatment with lycopene, and changes in the fraction of cells retained in different cell cycle phases. Lycopene promoted also cell cycle arrest followed by decreased cell viability in majority of cell linesafter 96 h, as compared to controls. Furthermore, an increase in apoptosis was observed in four cell lines (T-84, HT-29, MCF-7 and DU145) when cells were treated with lycopene. Our findings show the capacity of lycopene to inhibit cell proliferation, arrest cell cycle in different phases and increase apoptosis, mainly in breast, colon and prostate lines after 96 h. These observations suggest that lycopene may alter cell cycle regulatory proteins depending on the type of cancer and the dose of lycopene administration. Taken together, these data indicated that the antiproliferative effect of lycopene was cellular type, time and dose-dependent.lycopene, cancer, bioactive compounds, cell cycle.


PMID: 22866768

DOI: 10.1186/1475-2867-12-36

Other references

Albrecht, M., 1954: Cromatin-free division of cytoplasms of promyelocytes and myelocytes under the influence of mitotic poisons in vitro. Zeitschrift für Krebsforschung 60(1): 16-18

Barnes J.K., 1984: Biology and immature stages of dryomyza anilis diptera dryomyzidae. Information is presented on the life cycle of D. anilis Fallen, the saprophagous larvae of which feed on decaying animal matter and decaying fungi. Data on the habitat, behavior, feeding habits, and phenology of adults and immature stages are pres...

Weusten, A.Y.D.mir; Kaufmann, P.; Huppertz, B., 1999: Distribution of fibronectin isoforms and matrix metalloproteinases in extracellular matrices of the human placenta. Placenta 20(5-6): A 21, July-Aug

Borochov-Neori, H.; Judeinstein, S.; Harari, M.; Bar-Ya'akov, I.; Patil, B.S.; Lurie, S.; Holland, D., 2011: Climate effects on anthocyanin accumulation and composition in the pomegranate (Punica granatum L.) fruit arils. Worldwide pomegranate (Punica granatum L.) production has expanded greatly due to recent evidence on the fruit health attributes. The fruit's unique red color, conferred by anthocyanins, is an imperative sensory quality. Climate effects on th...

Sarkisov, A.K.; Dzhilavyan, K. A.; Ezhov, V.I.; Parfenov, I.S., 1967: Therapeutic and prophylactic efficacy of dibiomycin and ditetracycline in diseases of pigs and poultry. After a single i/m injection of 50, 000-100, 000 units/kg of dibiomycin to calves, piglets, ducks and chickens the antibiotic was detectable in the blood for up to 12-14 days. A single dose of dibiomycin and ditetracycline was 100% effective in ex...

Nuzzo, V.; Lain, O.; Giulivo, C., 1988: Techniques and experimental approaches in kiwi plant water-relations. Data are graphically presented from trials on water potential and its components in cv. Hayward leaves, assessed by the pressure-chamber, psychrometer-thermocouple, and cryoscopic-osmometer techniques. It was concluded that leaf osmotic potential...

Corso, J.J.; Yuille, A.; Sicotte, N.L.; Toga, A., 2007: Detection and segmentation of pathological structures by the extended graph-shifts algorithm. We propose an extended graph-shifts algorithm for image segmentation and labeling. This algorithm performs energy minimization by manipulating a dynamic hierarchical representation of the image. It consists of a set of moves occurring at different...

Stepanov, A.V.; Filimonov, G.P.; Zavodnov, V.Ia., 1988: A method of retrograde cholangiography. Vestnik Rentgenologii i Radiologii: 70-72

Seligmann, C.; Schimmer, M.; Leitsch, T.; Bock, A.; Simsek, Y.; Tschoepe, C.; Schultheiss, H.P., 2000: A thrombocyte-induced myocardial dysfunction in the ischemic and reperfused guinea pig heart is mediated by reactive oxygen species. In recent investigations, we could demonstrate that thrombocytes are able to contribute to ischemia- and reperfusion-induced injury of the heart. The aim of the current study was to investigate whether reactive oxygen species are responsible for i...

Schuster, Wilhelm, 1906: Dentsche insbesondere hessische Tiernamen. Zool, Frankfurt a. M: 173-176, 216-218