geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Biotransformation of benzene and toluene to catechols by phenol hydroxylase from Arthrobacter sp W


, : Biotransformation of benzene and toluene to catechols by phenol hydroxylase from Arthrobacter sp W.

Phenol hydroxylase gene engineered microorganism (PHIND) was used to synthesize catechols from benzene and toluene by successive hydroxylation reaction. HPLC-MS and 1H NMR analysis proved that the products of biotransformation were the corresponding catechols via the intermediate production of phenols. It was indicated that the main products of toluene oxidation were o-cresol and p-cresol. 3-Methylcatechol was the predominant product for m-cresol biotransformation. Formation rate of catechol (25 ?M/min/g cell dry weight) was 1.43-fold higher than that of methylcatechols. It was suggested that phenol hydroxylase could be successfully used to transform both benzene and toluene to catechols by successive hydroxylation.

US$29.90


Other references

Erasmus L.J.; Botha P.M.; Lebzien P.; Meissner H.H., 1990: Composition and intestinal digestibility of rumen fermented feeds and duodenal digesta in cows using bag techniques. Journal of Dairy Science. 73(12): 3494-3501

Buerger, L.; Rohr, H., 1960: Generalized necrotizing angiitis with symmetrical renal cortex necrosis in pregnancy following a vericella infection (A contribution to the problem of symmetrical renal cortex necrosis). Deutsches Archiv für Klinische Medizin 206: 305-321

Shastry, P.P., 1995: Ecoclimatic conditions for the development of new wilt of cotton. In the 1989 and 1991 the ecoclimatic conditions that favoured the development of New Wilt were higher maximum temperature (30-35 degree C), higher mean temperature (26-30 degree C), lower amount of precipitation (4-22 mm) with higher number of dry...

Anonymous, 1979: Economics of fertilizer use in selected developing countries. The analysis concerns certain developing countries covered by the FAO fertilizer programme: Bangladesh, Indonesia, Pakistan and the Philippines. Factors such as fertilizer price situation, crop-price situation, value-constructions and return on fe...

Chakraborty, A., 2003: Noonan syndrome: a brief overview. Noonan syndrome is a relatively common dysmorphic syndrome. The typical features include short stature, cardiovascular abnormalities and a characteristic facies. Children with Noonan syndrome have a considerable number of potential health problems...

Kovaleva N.V., 1981: New data on a black sea microbial population. Soviet Journal Of: 20-24

Carignan, A.; Sherman, S., 1991: Nurse education partnerships benefit facilities, colleges. Provider 18(1): 33-34

Nriagu J.O.; Hem J.D., 1978: Chemistry of pollutant sulfur in natural waters. Pages 211-270 1978

Chamber, F.W., 1963: Miniature tissue equivalent ionization chambers and their use. Aerospace Medicine 34: 193-196

Ballehaninna, U.K.; Chamberlain, R.S., 2014: Utility of intraoperative frozen section examination of sentinel lymph nodes in ductal carcinoma in situ of the breast. Intraoperative frozen section (IFS) examination of sentinel lymph nodes (SLN) is useful in selecting patients with invasive breast cancer for immediate axillary lymph node dissection. However, whether IFS evaluation of the SLNs in ductal carcinoma...