geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Rab31 expression levels modulate tumor-relevant characteristics of breast cancer cells


, : Rab31 expression levels modulate tumor-relevant characteristics of breast cancer cells. Molecular Cancer 11: 62-62

Rab proteins constitute a large family of monomeric GTP-binding proteins that regulate intracellular vesicle transport. Several Rab proteins, including rab31, have been shown to affect cancer progression and are related with prognosis in various types of cancer including breast cancer. Recently, the gene encoding rab31 was found to be overexpressed in estrogen receptor-positive breast cancer tissue. In a previous study we found a significant association of high rab31 mRNA expression with poor prognosis in node-negative breast cancer patients. In the present study, we aimed to investigate the impact of rab31 (over)-expression on important aspects of tumor progression in vitro and in vivo. Breast cancer cells displaying low (MDA-MB-231) or no (CAMA-1) endogenous rab31 expression were stably transfected with a rab31 expression plasmid. Batch-transfected cells as well as selected cell clones, expressing different levels of rab31 protein, were analyzed with regard to proliferation, cell adhesion, the invasive capacity of tumor cells, and in vivo in a xenograft tumor model. Polyclonal antibodies directed to recombinantly expressed rab31 were generated and protein expression analyzed by immunohistochemistry, Western blot analysis, and a newly developed sensitive ELISA. Elevated rab31 protein levels were associated with enhanced proliferation of breast cancer cells. Interestingly, weak to moderate overexpression of rab31 in cell lines with no detectable endogenous rab31 expression was already sufficient to elicit distinct effects on cell proliferation. By contrast, increased expression of rab31 in breast cancer cells led to reduced adhesion towards several extracellular matrix proteins and decreased invasive capacity through MatrigelTM. Again, the rab31-mediated effects on cell adhesion and invasion were dose-dependent. Finally, in a xenograft mouse model, we observed a significantly impaired metastatic dissemination of rab31 overexpressing MDA-MB-231 breast cancer cells to the lung. Overexpression of rab31 in breast cancer cells leads to a switch from an invasive to a proliferative phenotype as indicated by an increased cell proliferation, reduced adhesion and invasion in vitro, and a reduced capacity to form lung metastases in vivo.

US$19.90

PMID: 22920728

DOI: 10.1186/1476-4598-11-62


Other references

Provenzano, G., 2005: Discussion of analytical equation for variation of discharge in drip irrigation laterals by V. Ravikumar, C.R. Ranganathan, and S. Santhana Bosu. Journal of irrigation and drainage engineering e 131(3): 300-302

Reed D.; Yano K., 1991: Predictors of arteriographically defined coronary stenosis in the honolulu heart program. The purpose of this study was to determine if the major risk factors for clinical myocardial infarction also predicted coronary artery stenosis as defined by arteriography. Of a cohort of 7,591 men who were free of cardiovascular disease at entry,...

Greene, J., 1994: Report examines docs. Modern Healthcare 24(38): 20-21

Di-Ilio, C.; Polidoro, G.; Arduini, A.; Muccini, A.; Federici, G., 1983: Glutathione peroxidase ec 1.11.1.9 glutathione reductase ec 1.6.4.2 glutathione s transferase ec 2.5.1.18 and gamma glutamyl trans peptidase ec 2.3.2.2 activities in the human early pregnancy placenta. GSH [glutathione] peroxidase, GSSG [glutathione] reductase, GSH S-transferase, and .gamma.-glutamyltranspeptidase activities were measured in the supernatant of 13 human early pregnancy placenta homogenates. From measurements of GSH peroxidase act...

Diefenbach, C., 1997: Muscle relaxants and the heart muscle. Der Anaesthesist 46(9): 807-807

Golding, D.L.; Hall, O.F., 1961: Tests of precision of cubic-foot tree-volume equations. A test of precision of 25 equations was made for each of three species-Pinus banksiana, Picea glauca, and Populus tremuloides. Considering the independent variables of d.b.h. and height only, the equation that combines the highest precision and ea...

Ly, K., 2010: Unlocking CNN careers. Community Practitioner 82(10): 12-13

Feld, O.; Yahalom, G.; Livneh, A., 2013: Neurologic and other systemic manifestations in FMF: published and own experience. Familial Mediterranean fever (FMF) is an autosomal recessive autoinflammatory disease, presenting with recurrent episodes of fever and polyserositis. Neurologic involvement in FMF is rare and usually considered fortuitous. The aim of this article...

Tofield, A., 2015: Warfarin increases stroke risk in atrial fibrillation. European Heart Journal 35(22): 1421-1421

Chaudru, Vérie.; Lo, M.T.; Lesueur, F.; Marian, C.; Mohamdi, H.; Laud, K.; Barrois, M.; Chompret, A.; Avril, M.F.; Demenais, F.; Bressac-de Paillerets, B., 2010: Protective effect of copy number polymorphism of glutathione S-transferase T1 gene on melanoma risk in presence of CDKN2A mutations, MC1R variants and host-related phenotypes. The effect of CDKN2A, the major high-risk melanoma susceptibility gene, has been shown to be modified by host-related phenotypes and variants of MC1R gene. The glutathione S-transferase (GSTs) genes, implicated in detoxification of metabolites aft...