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Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q


, : Expression of human growth hormone in the milk of transgenic rabbits with transgene mapped to the telomere region of chromosome 7q. Journal of Applied Genetics 53(4): 435-442

The advent of transgenic technology has provided methods for the production of pharmaceuticals by the isolation of these proteins from transgenic animals. The mammary gland has been focused on as a bioreactor, since milk is easily collected from lactating animals and protein production can be expressed at very high levels, including hormones and enzymes. We demonstrate here the expression pattern of recombinant human growth hormone (rhGH) in transgenic rabbits carrying hGH genomic sequences driven by the rat whey acidic protein (WAP) promoter. The transgene was mapped to the q26-27 telomere region of chromosome 7q by fluorescence in situ hybridization (FISH). Nearly 30 % of the F1 generation demonstrated the presence of transgene. The recombinant growth hormone was detected in the milk of the transgenic rabbit females, but not in serum, up to the level of 10 μg/ml. Ectopic expression of the transgene in the brain, heart, kidney, liver, and salivary gland was not observed, indicating that a short sequence of rat WAP promoter (969 bp) contained essential sequences directing expression exclusively to the mammary gland. The biological activity of recombinant growth hormone was measured by immunoreactivity and the capability to stimulate growth of the hormone-dependent Nb211 cell line.

US$19.90

PMID: 22898896

DOI: 10.1007/s13353-012-0110-4


Other references

Pinilla, A.C.talan, J., 1970: Sedimentological study of the Aragonese zone of the Tertiary basin of the Ebro. Anales de edafologia y agrobiologia: 29 (11 12) 957-972

Uehara, Y.; Taguchi Hakamada, R.; Kawabata, Y.; Hirawa, N.; Goto, A.; Omata, M., 2000: The role of Y chromosome from Dahl salt-sensitive rats on development of salt-dependent hypertension. Journal of Hypertension 18(Suppl 4): S125

Zong, Y.; Wu, J-yi.; Shen, K-wei., 2014: The Interpretation consensus recommendations on primary systemic therapy in the management of early breast cancer. Zhonghua Wai Ke Za Zhi 51(1): 10-13

Wolfson, N.E.; Kraiger, K., 2014: Cognitive aging and training: the role of instructional coherence and advance organizers. BACKGROUND/STUDY CONTEXT: This study investigates whether there is a need for age-specific computer-based instructional design. The authors examined the effect of two design principles, instructional coherence and advance organizers, on learning o...

Morales, C.; André, L.; Adler, L., 1990: A procedure for enrichment and isolation of mutants of the salt-tolerant yeast Debaryomyces hansenii having altered glycerol metabolism. The salt-tolerant yeast Debaryomyces hansenii produces and accumulates glycerol when subjected to salt stress, whereby the buoyant density of the cells is changed. This property allows for enrichment of mutants with altered glycerol metabolism by...

Park, C.; Ma, Y.D.; Choi, K., 2005: Evidence for the hemangioblast. In recent years, numerous cellular and genetic studies have led to a better understanding of the developmental relationship between hematopoietic and endothelial cell lineages. Specifically, tracing cells expressing various genes such as Brachyury...

Joklik, W.K., 1977: Evidence for a translation-inhibitory ribosome-associated protein. Texas Reports on Biology and Medicine 35: 264-269

Rao, V.R.; Clarenburg, R., 1977: Pharmacokinetics of intravenously injected chloramphenicol in baby pigs. A sensitive fluorometric assay procedure for chloramphenicol and its metabolites was used to measure distribution in tissues and elimination of the drug after injection of 22 mg/kg iv in baby pigs. Disappearance of chloramphenicol from plasma was...

Ghose M.; Johri B.M., 1987: Cell inclusions in vegetative structure of young palms. The occurrence and types of cell inclusions, or ergastic substances, in 19 species of seedling palms are described. Two types of silica bodies occur in these palms .sbd. 'hat-shaped or conical' type and 'spherical or druse-like&apos...

Griffith, A.V.; Fallahi, M.; Nakase, H.; Gosink, M.; Young, B.; Petrie, H.T., 2010: Spatial mapping of thymic stromal microenvironments reveals unique features influencing T lymphoid differentiation. Interaction of hematopoietic progenitors with the thymic microenvironment induces them to proliferate, adopt the T lineage fate, and asymmetrically diverge into multiple functional lineages. Progenitors at various developmental stages are stratifi...