geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

An exploratory clinical study on the safety and efficacy of an autologous fibroblast-seeded artificial skin cultured with animal product-free medium in patients with diabetic foot ulcers


, : An exploratory clinical study on the safety and efficacy of an autologous fibroblast-seeded artificial skin cultured with animal product-free medium in patients with diabetic foot ulcers. International Wound Journal 11(2): 183-189

Cultured dermal substitutes have been used for the treatment of chronic skin ulcers; however, the biological risks of animal-derived materials in the culture process such as foetal bovine serum (FBS) have been reported. In this study, we prepared an autologous fibroblast-seeded artificial dermis (AFD) using animal-product-free medium supplemented with 2% patient autologous serum and without any animal-derived materials such as trypsin in the culturing process. We applied the AFD in five patients with diabetic ulcers and investigated its safety and efficacy. As the primary endpoint, we defined 'wound bed improvement' according to the percentage of granulation area to the whole wound area on day 21, and 60% or higher was regarded as improved. The mean age of the patients was 60·6 years and the mean duration of the ulcer was 22·6 months. In the evaluation of the primary endpoint, the 'wound bed' was improved in all patients [proportion of improvement: 100%, 95% confidence interval (CI): 48% to 100%]. Three patients had complete wound healing within 12 weeks after application and two patients had >80% wound healing at 12 weeks. Side effects were not serious. Our AFD may be a safe and effective treatment of diabetic ulcers.

US$19.90

PMID: 22958543

DOI: 10.1111/j.1742-481X.2012.01064.x


Other references

Maury, M.; Walti, H.; Richer, C.; Francoual, C.; Relier, J.P.; Giudicelli, J.F., 1984: Serum angiotensin I-converting enzyme activity in premature and full-term infants. Serum angiotensin I-converting enzyme activity (SCEA) was investigated in 15 healthy premature (gestational age ranging from 30 to 36 weeks) and in 14 healthy full-term (gestational age ranging from 37 to 41 weeks) newborns as well as in 16 health...

Sanz-Ortiz, J., 2004: Anorexia treatment in the oncological patient. Revista Clinica Espanola 204(10): 542-544

Flute, P.T., 1964: Haemorrhage And Fibrinolysis. Proceedings of the Royal Society of Medicine 57: 603-606

Parés, X.; Farrés, J.; Kedishvili, N.; Duester, G., 2008: Medium- and short-chain dehydrogenase/reductase gene and protein families : Medium-chain and short-chain dehydrogenases/reductases in retinoid metabolism. Retinoic acid (RA), the most active retinoid, is synthesized in two steps from retinol. The first step, oxidation of retinol to retinaldehyde, is catalyzed by cytosolic alcohol dehydrogenases (ADHs) of the medium-chain dehydrogenase/reductase (MDR...

de Coo, R.F.; Buddiger, P.A.; Smeets, H.J.; van Oost, B.A., 1999: The structure of the human NDUFV1 gene encoding the 51-kDa subunit of mitochondrial complex I. The genomic organization of the human 51-kDa subunit gene (NDUFV1) on human Chromosome (Chr) 11q13 was determined. The NDUFV1 gene consists of 10 exons. Exon 1 encodes for the 20-amino-acids-long import sequence, and exon 1 through 10 codes for th...

Bomalaski, J.S., 1990: Acute rheumatologic disorders in the elderly. A variety of rheumatologic disorders affect the elderly. Some of these problems are seen almost exclusively in the elderly, such as temporal arteritis and pseudogout. Because of underlying chronic diseases, these patients are also at increased ris...

Van Marle G.W.R.B.; Gommers P.A.M., 1982: Digitalized echo oculometry. Thijssen, J M And A M Verbeek (Ed ) Documenta Ophthalmologica Proceedings Series, Vol 29 Ultrasonography in Ophthalmology; Proceedings Of The 8th Siduo (Societas Internationalis Pro Diagnostica Ultrasonica in Ophthalmologia) Congress, Nijmegen, Netherlands Xiv+538p Dr W Junk Bv Publishers: The Hague, Netherlands Boston, Mass , Usa (Dist in The Usa By Kluwer Boston, Inc : Hingham, Mass ) Illus P521-526, 1981

Bramucci, M.G.; Dicosimo, R.; Fallon, R.; Gavagan, J.E.; Herkes, F.; Wilczek, L., 2003: 3-hydroxycarboxylic acid production and use in branched polymers. This invention relates to a process for the preparation of a 3-hydroxycarboxylic acid from a 3-hydroxynitrile. More specifically, 3-hydroxyvaleronitrile is converted to 3-hydroxyvaleric acid in high yield at up to 100% conversion, using as an enzy...

Brown, J.; White, D.A.E.; Magnus, M.; Czarnogorski, M.; Lee, V., 2010: CD4 counts in patients diagnosed with HIV through routine HIV screening in two urban emergency departments. Journal of Acquired Immune Deficiency Syndromes 54(5): E15-E16

Baryluk, M.; Oblonczek, G.; Zolmowski, J., 1977: Injury of the semilunar cartilages of the knee-joint in childhood. In the paper, 30 patients at the age of 5 till 14 with injuries of the semilunar cartilages of the knee-joint, are presented. All the children were subjected to surgery, and 20 of them underwent same examinations performed in a delay of 1 to 12 ye...