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Phenotypes and clinical significance of circulating CD4(+)CD25(+) regulatory T cells (Tregs) in patients with acute-on-chronic liver failure (ACLF)


, : Phenotypes and clinical significance of circulating CD4(+)CD25(+) regulatory T cells (Tregs) in patients with acute-on-chronic liver failure (ACLF). Journal of Translational Medicine 10: 193-193

CD4+CD25+ regulatory T cells (Tregs) play an important role in maintaining immunological tolerance to self and foreign antigens. T cell receptors (TCR) reflect the composition and function of T cells. It is not universally agreed that there is a relationship between CD4+CD25+ Treg frequency and the severity of acute-on-chronic liver failure (ACLF). The repertoire of TCR beta chain variable (TCRBV) regions of peripheral Tregs in ACLF patients is not well understood. Human PBMCs were separated and sorted into CD4+CD25+ Treg subsets using density gradient centrifugation and magnetic activated cell sorting (MACS). The CD4+CD25high Treg frequency in peripheral blood of ACLF and chronic hepatitis B (CHB) patients was measured by flow cytometry. The molecular profiles of TCRBV CDR3 were determined using gene melting spectral pattern (GMSP) analysis. TCRBV gene families were cloned and sequenced when the GMSP profiles showed a single-peak. CD4+CD25high Treg prevalence in peripheral blood of ACLF patients is increased significantly compared to healthy donors (HDs) (P < 0.01) and CHB patients (P < 0.01). The prevalence of CD4+CD25high Tregs in ACLF or CHB patients is positively correlated with HBV DNA load. The TCRBV11, BV13.1, BV18, BV20 are the most prevalent TCRBV in CD4+CD25+ Tregs in ACLF and CHB patients. In addition, the CDR3 motifs were relatively conserved in these four TCRBV gene families. The CD4+CD25high Tregs prevalence in peripheral blood is indicative of disease severity in ACLF or CHB patients. The relatively conserved TCRBV20 CDR3 motif "TGTGHSPLH" and TCRBV11 CDR3 motif "VYNEQ" may be used in helping diagnosis and treat patients with ACLF.

US$19.90

PMID: 22978653

DOI: 10.1186/1479-5876-10-193


Other references

Lance, M.R.; Washington, C.; Davis, S.S., 1996: Evidence for the formation of amphotericin B-phospholipid complexes in Langmuir monolayers. To study the interaction of the polyene antifungal amphotericin B with phospholipid Langmuir monolayers and to correlate with stability of phospholipid-stabilized drug emulsions. Pressure-area isotherms of mixed monolayers of amphotericin B (0-20...

Fossdal, R.; Magnússon, L.; Weber, J.L.; Jensson, O., 1995: Mapping the locus of atrophia areata, a helicoid peripapillary chorioretinal degeneration with autosomal dominant inheritance, to chromosome 11p15. Atrophia areata (AA) is an early onset autosomal dominant helicoid peripapillary chorioretinal degeneration, which was first demonstrated to be hereditary in an Icelandic family. It is characterized by bilateral wing-shaped atrophic areas of the r...

Khubetsova, R.D., 1955: The problem of the mechanism of the Schwartzman phenomenon Communication II The effect of a local injection of novocaine on the course of the Schwartzman phenomenon Referat Zhur, Biol, 1956, No 74472. Three ml of an aqueous solution of purified complete intestinal bacillus antigen, prepared according to a modification of the method of Revo-Buaven [Boivin?], was injected intradermally into rabbits in 2 groups. On the following day, a decisive do...

Pape, K.E., 1981: Intraventricular hemorrhage: diagnosis and outcome. Birth Defects Original Article Series 17(1): 143-151

Budin, K.S., 1999: Intraspecific variability and gene centers of selection of phytophthora resistant species of Solanum genus Petota section. The results of genetic investigations of intraspecific polymorphism on phytophthora resistance among wild species of Solanum genus were presented. It was determined four gene centers of formation of genotypes resistant to pathogene. It was establi...

Lund, P.C., 2014: The physiology of refrigeration anesthesia. Manitoba Medical Review 27(3): 141-141

Kleinsorge, H., 1964: Allergy--A Psychosomatic Disease?. Medizinische Klinik 59: 1193-1195

Messerklinger, W., 1981: Endoscopy of the nose in diseases of the lacrimal apparatus. Bucherei des Augenarztes: 14-18

Davidson, T.; Klang, E.; Goshen, E.; Goldstein, J.; Khaikin, M.; Chikman, B.; Ben-Haim, S., 2017: Postoperative changes after surgical mesh hernia repair: a pitfall in interpretation of 18F-FDG PET-CT. A number of case reports have demonstrated FDG uptake around mesh prostheses after hernia repair surgery. This study characterizes FDG PET-CT findings after hernia repair with synthetic mesh in a series of cancer patients. FDG PET-CT studies were...

Karataş, Aşegül.; Yüksel, Nüfer.; Baykara, T., 2005: Improved solubility and dissolution rate of piroxicam using gelucire 44/14 and labrasol. Piroxicam is a nonsteroidal anti-inflammatory drug that is characterized by low solubility-high permeability. The present study was designed to improve the dissolution rate of piroxicam at the physiological pH's through its increased solubili...