geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Monoallelic gene targeting in hypoblast stem cells reveals X chromosome inactivation


, : Monoallelic gene targeting in hypoblast stem cells reveals X chromosome inactivation. Biochemical and Biophysical Research Communications 427(3): 563-567

We recently isolated hypoblast stem cells (HypoSC), which are related to embryonic stem (ES) cells. ES cells efficiently perform homologous recombination (HR) and lack X chromosome inactivation (Xi), but it is unknown whether the same applies to HypoSC. Using the X-linked hypoxanthine phosphoribosyl transferase (HPRT) gene, we find that HypoSC perform HR with similar frequency as ES cells. Monoallelic targeting in female HypoSC eliminated HPRT gene expression, implying epigenetic inactivation of the other allele. Although density-induced differentiation complicated selection, the targeted clones maintained their original properties. These results will facilitate targeted genetic manipulation of HypoSC and the study of Xi. We demonstrate homologous recombination (HR) in hypoblast stem cells (HypoSC). We targeted the X-linked hypoxanthine phosphoribosyl transferase (HPRT) gene. Targeting efficiency was high, similar to that seen in embryonic stem cells. In female HypoSC, the non-targeted HPRT allele was not expressed. Thus HypoSC efficiently perform HR and likely exhibit X chromosome inactivation.

US$19.90

PMID: 23022182

DOI: 10.1016/j.bbrc.2012.09.097


Other references

Krone, H.A.; Probst, E., 1967: Ovarian tumor and pregnancy. Zentralblatt für Gynakologie 89(52): 1905-1910

Stein, I., 2011: Finder and screw driver used in driving screws with a drill. American Journal of Surgery 74(3): 380-380

Conzo, G.; Calò, P.Giorgio.; Gambardella, C.; Tartaglia, E.; Mauriello, C.; Della Pietra, C.; Medas, F.; Santa Cruz, R.; Podda, F.; Santini, L.; Troncone, G., 2015: Controversies in the surgical management of thyroid follicular neoplasms. Retrospective analysis of 721 patients. The most appropriate surgical management of "follicular neoplasm/suspicious for follicular neoplasm" lesions, is still controversial. Analysing and comparing the experience of two units for endocrine surgery, we retrospectively evaluated...

Zock, J.P.; Sunyer, J.; Kogevinas, M.; Kromhout, H.; Burney, P.; Antó, J.M., 2001: Occupation, chronic bronchitis, and lung function in young adults. An international study. We studied the relationship between occupational exposures, chronic bronchitis, and lung function in a general population survey in 14 industrialized countries, including 13,253 men and women aged 20 to 44 yr. We studied associations between occup...

Charlier, T.D.; Ball, G.F.; Balthazart, J., 2006: Plasticity in the expression of the steroid receptor coactivator 1 in the Japanese quail brain: effect of sex, testosterone, stress and time of the day. Analysis of nuclear receptor action on the eukaryotic genome highlights the importance of coactivators on gene transcription. The steroid receptor coactivator-1 in particular is the focus of an intense research and physiological or behavioral stud...

Hsiao, W-Tien.; Lin, L-Hsiang.; Chiang, H-Jen.; Ou, K-Liang.; Cheng, H-Yi., 2015: Biomedical electrosurgery devices containing nanostructure for minimally invasive surgery: reduction of thermal injury and acceleration of wound healing for liver cancer. The aim of the present study was to investigate the thermal injury in the liver after a minimally invasive electrosurgery technique with a copper-doped diamond-like carbon (DLC-Cu) surface coating. To effectively utilize electrosurgery in a clinic...

Lancaster, J.L., 1957: Preliminary studies of granular insecticides against the lone star tick. The use of granular dieldrin as a means of controlling ticks in wooded areas may be practical as shown by preliminary investigations in the greenhouse.

Edwards, A.W.F., 1974: On Kimura's maximum principle in the genetical theory of natural selection. For a single locus with k alleles A1, .., Ak, frequencies p1, .., pk, and viability wij of the genotype AiAj (= AjAi), Kimura's principle states that, under random mating, natural selection causes the gene frequencies to change from [pi] in o...

Hawrot, R.Y.; Niemi, G.J., 1996: Effects of edge type and patch shape on avian communities in a mixed conifer-hardwood forest. Forest bird communities were sampled along line transects in northwestern Wisconsin during June of 1985 through 1992 to determine whether edge type and patch shape affect avian abundance. Landscape structural characteristics quantified along these...

Barbero, Héctor.; Ferrero, S.; Álvarez-Miguel, Lía.; Gómez-Iglesias, P.; Miguel, D.; Álvarez, C.M., 2016: Affinity modulation of photoresponsive hosts for fullerenes: light-gated corannulene tweezers. NlmCategory="UNASSIGNED">Six azobenzene derivatives bearing polyaromatic fragments have been prepared and their reversible photoisomerization has been assessed. Corannulene-functionalized molecules have demonstrated excellent switchab...