geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Lipopolysaccharide promotes lipid accumulation in human adventitial fibroblasts via TLR4-NF-kappaB pathway


, : Lipopolysaccharide promotes lipid accumulation in human adventitial fibroblasts via TLR4-NF-kappaB pathway.

Atherosclerosis is a chronic degenerative disease of the arteries and is thought to be one of the most common causes of death globally. In recent years, the functions of adventitial fibroblasts in the development of atherosclerosis and tissue repair have gained increased interests. LPS can increase the morbidity and mortality of atherosclerosis-associated cardiovascular disease. Although LPS increases neointimal via TLR4 activation has been reported, how LPS augments atherogenesis through acting on adventitial fibroblasts is still unknown. Here we explored lipid deposition within adventitial fibroblasts mediated by lipopolysaccharide (LPS) to imitate inflammatory conditions. In our study, LPS enhanced lipid deposition by the up-regulated expression of adipose differentiation-related protein (ADRP) as the silencing of ADRP abrogated lipid deposition in LPS-activated adventitial fibroblasts. In addition, pre-treatment with anti-Toll-like receptor 4 (TLR4) antibody diminished the LPS-induced lipid deposition and ADRP expression. Moreover, LPS induced translocation of nuclear factor-kappaB (NF-kappaB), which could markedly up-regulate lipid deposition as pre-treatment with the NF-kappaB inhibitor, PDTC, significantly reduced lipid droplets. In addition, the lowering lipid accumulation was accompanied with the decreased ADRP expression. Furthermore, LPS-induced adventitial fibroblasts secreted more monocyte chemoattractant protein (MCP-1), compared with transforming growth factor-beta1 (TGF-beta1). Taken together, these results suggest that LPS promotes lipid accumulation via the up-regulation of ADRP expression through TLR4 activated downstream of NF-kappaB in adventitial fibroblasts. Increased levels of MCP-1 released from LPS-activated adventitial fibroblasts and lipid accumulation may accelerate monocytes recruitment and lipid-laden macrophage foam cells formation. Here, our study provides a new explanation as to how bacterial infection contributes to the pathological process of atherosclerosis.

US$29.90


Other references

Cerutis, D.R.; Hass, N.A.; Iversen, L.J.; Bylund, D.B., 1994: The cloning and expression of an OK cell cDNA encoding a 5-hydroxytryptamine1B receptor. Serotonin (5-hydroxytryptamine or 5-HT) is an important biogenic amine that functions as both a neurotransmitter and a hormone in the central nervous system (CNS) and the periphery. We report here the isolation of a cDNA from the OK cell that enco...

Bonfitto, V.L.; Demasi, A.P.; Costa, A.F.; Bonfitto, J.F.; Araujo, V.C.; Altemani, A., 2011: High-grade transformation of adenoid cystic carcinomas: a study of the expression of GLUT1 glucose transporter and of mitochondrial antigen. To broaden understanding of phenomena involved in progression from classical adenoid cystic carcinomas (ACCs) to tumours with high-grade transformation (ACC-HGT) METHODS: Expression of proteins linked to cellular metabolism as well as the microvas...

Pinkstaff, Carlin A., 1964: Hiatochemical studies on prenatal, neonatal and adult rat kidney. Anat Rec 148(2): 322-323

Close, R.; Hoh, J.F., 1967: Force: velocity pproperties of kitten muscles. 1. The characteristics of isometric contractions and force:velocity properties of the extensor digitorum longus (EDL) and soleus (SOL) muscles of neonatal kittens were determined in situ.2. The mean contraction time is 51 msec for EDL and 70 msec...

Rabega, C.; Dumitrescu, F.; Rabega, M.; Stanescu, R., 1977: The lipid spectra in some forms of hereditary dys lipidosis. The variation of lipid fractions in familial hypercholesterolemia was studied. Total cholesterol, triglycerides, total lipids and .beta.-lipoproteins in the blood of 76 subjects with cardiovascular disorders, and their descendants, were examined....

Wosilait, W.D.; Soler-Argilage, C.; Nagy, P., 1976: A theoretical analysis of the binding of palmitate by human serum albumin. The distribution of palmitate between the form bound by human serum albumin and the free form in plasma was calculated by use of 12 stepwise equilibrium constants and a computer program. Computations were carried out for molar ratios of palmitate...

Eckford, S.; Lake, Y., 1997: First trimester traumatic tetraplegia-management of pregnancy and delivery. Journal of Obstetrics and Gynaecology 17(1): 59-60

Geiser, F.; McAllan, B.M.; Kenagy, G.J.; Hiebert, S.M., 2006: Photoperiod affects daily torpor and tissue fatty acid composition in deer mice. Photoperiod and dietary lipids both influence thermal physiology and the pattern of torpor of heterothermic mammals. The aim of the present study was to test the hypothesis that photoperiod-induced physiological changes are linked to differences i...

Torpisch, K., 1951: Pain alleviation in normal labor. Zeitschrift für Klinische Medizin 2(7-8): 179-182

Folk C., 1967: Post natal development of the jackdaw corvus monedula under natural conditions. Zoologicke Listy 16(4): 379-393