geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Is cystathionine gamma-lyase protein expressed in the heart?


, : Is cystathionine gamma-lyase protein expressed in the heart?. Biochemical and Biophysical Research Communications 428(4): 469-474

Hydrogen sulfide (H2S) has emerged as an important gasotransmitter, offering protection against ischemia-reperfusion damage to the heart. Cystathionine gamma-lyase (CSE) is believed to be the major H2S-generating enzyme in the heart. Quite contrary to the general contemplation, CSE protein in cardiac tissues has not been convincingly detected and it has become an issue of controversy. In the present study, we isolated cardiac tissues from wild type (WT) and CSE knockout [1] mice or the rat. CSE expression at transcriptional and translational levels were assayed by RT-PCR and Western blotting with five different antibodies (four commercial products and one homemade), respectively. Cardiac H2S production rate was also examined. Our data validated the expression of CSE mRNA in the heart of WT mice or rats, not in CSE KO mice. Using all 5 different anti-CSE antibodies, we could not detect CSE proteins in mouse or rat cardiac tissues or in cultured rat cardiomyocytes. On the other hand, CSE protein was detectable in liver tissues from WT mice with the expected molecular mass of 43.6 kDa. H2S production rate of heart tissues in CSE KO mice was significantly decreased compared with that in WT mice. In the presence of an CSE inhibitor, D,L-propargylglycine, H2S production rate of heart tissues from WT mice was inhibited by approximately eighty percent. It appears that CSE mediates mostly endogenous H2S production in heart tissues. However, the available anti-CSE antibodies could not detect CSE proteins in rat and mouse heart tissues or rat cardiomyocytes. Hydrogen sulfide (H2S) protects the heart from ischemia/reperfusion damage. Cardiac production of H2S is mainly mediated by cystathionine gamma-lyase (CSE). Whether CSE protein is expressed in the heart is controversial. Using 5 different anti-CSE antibodies,no CSE can be detected in rat or mouse heart. The role of CSE-produced H2S in the heart should be re-evaluated.

US$19.90

PMID: 23122812

DOI: 10.1016/j.bbrc.2012.10.084


Other references

Studier, E.H.; Procter, J.W., 1971: Respiratory gases in burrows of Spermophilus tridecemlineatus. Journal of Mammalogy, 52: 631-633

Prieto, CE.; Gomez, BJ., 1991: Las areas de distribucion de los Helicoideos (Gastropoda, Pulmonata) del Pais Vasco y regiones adyacentes. 2. Familia Hygromiidae (salvo generos Helicelloides). Iberus. 1988( ); 82: 9-14

Mukhopadhyay, N.; Fernbach, D.J.; Mumford, D.M.; South, M.A., 1978: T and B cells and immune competence in human fetal liver cells. Clinical Immunology and Immunopathology 10(1): 59-64

Lobotka, J., 1977: Transport and rationing of grain feed for cattle. Mechanizace zemedelstvi: 7 (10) 447-450

Vlasov, V.A.; Kalachikov, S.M.; Dymshits, G.M., 1994: Synthesis of fluorescent labelled uridine 5'-triphosphate derivatives for preparation of nonradioactive RNA probes. Synthesis of uridine 5'-triphosphate derivatives bearing residues of fluorescent dyes (fluorescein and rhodamine) at the terminus of a spacer at C5 of pyrimidine ring is described. The influence of the reaction conditions on the yield of the...

Hiller, H.G., 1955: Barium enema reduction of intussusception in infancy. Medical Journal of Australia 42(5): 157-157

Glaser, H., 1950: Reducing labour costs in power-saw logging by skidding. Gives the results of time studies in Beech forests in Hesse to demonstrate the advantages of skidding tree-length material, with minimum trimming at the felling site, to points where cross-cutting by power saw can be centralized.

Serban, Nm, 1980: Septate junction between visual cells of Drosophila subobscura eye. Biology: proceedings of the Seventh European Congress on Electron Microscopy The Hague The Netherlands August 24-29-1980 editors: P Brederoo and W de Priester: 1

Yokoyama I.; Yamanaka N.; Kato N.; Ichihashi H.; Hosoi M.; Ohta K.; Ichimura I.; Kondo T., 1985: Pelvic perfusion hyperthermia for advanced pelvic malignancies. Pelvic function hyperthermia (PPH) for malignant gynecologic diseases in the pelvis was performed for 4 h with favorable results. Serial monitoring of chemotherapeutic drug concentrations in the blood, tissues and circuit showed that the drugs wer...

Yang, G.; Peng, X.; Wu, Y.; Li, T.; Liu, L., 2017: Involvement of connexin43 phosphorylation and gap junctional communication in vasopressin-induced ROCK-dependent vasoconstriction after hemorrhagic shock. We examined the roles played by gap junctions (GJs) and the GJ channel protein connexin43 (Cx43) in arginine vasopressin (AVP)-induced vasoconstriction after hemorrhagic shock, and their relationship to Rho kinase (ROCK) and protein kinase C (PKC)...