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Ligand and pathogen specificity of the Atlantic salmon serum C-type lectin


, : Ligand and pathogen specificity of the Atlantic salmon serum C-type lectin. Biochimica et Biophysica Acta 1830(1): 2129-2138

An Atlantic salmon (Salmo salar) C-type lectin (SSL) binds to mannose and related sugars as well as to the surface of Aeromonas salmonicida. To characterize this lectin as a pathogen recognition receptor in salmon, aspects of its interaction with molecules and with intact pathogens were investigated. SSL was isolated using whole-yeast-affinity and mannan-affinity chromatography. The binding of SSL to the two major surface molecules of A. salmonicida, lipopolysaccharide (LPS) and A-layer protein was investigated by western blotting and enzyme-linked immunosorbent assays. Microbial binding specificity of SSL was examined by whole cell binding assays using a range of species. Carbohydrate ligand specificity of SSL was examined using glycan array analysis and frontal affinity chromatography. SSL showed binding to bacteria and yeast including, Pseudomonas fluorescens, A. salmonicida, A. hydrophila, Pichia pastoris, and Saccharomyces cerevisiae, but there was no detectable binding to Yersinia ruckeri. In antimicrobial assays, SSL showed no activity against Escherichia coli, Bacillus subtilis, S. cerevisiae, or A. salmonicida, but it was found to agglutinate E. coli. The major surface molecule of A. salmonicida recognized by SSL was shown to be LPS and not the A-layer protein. LPS binding was mannose-inhibitable. Glycans containing N-acetylglucosamine were shown to be predominant ligands. SSL has a distinct ligand preference while allowing recognition of a wide variety of related carbohydrate structures. General Significance SSL is likely to function as a wide-spectrum pattern recognition protein. Atlantic salmon serum lectin (SSL) recognizes specific bacteria and fungi. Laboratory analyses reveal SSL binding to A. salmonicida lipopolysaccharide. Glycan array-binding experiments show SSL binding to glycans with terminal N-acetylglucosamine.

US$19.90

PMID: 23032861

DOI: 10.1016/j.bbagen.2012.09.019


Other references

Kmietowicz, Z., 2016: Staffing levels are not to blame for worse obstetric outcomes at weekends, study finds. Bmj 351: H6324-H6324

Zhu Erqin; Wang Qi, 1988: Sedimentation on the north shelf of the East China Sea. A reconnaissance study has determined the overall pattern of sedimentation on the northern continental shelf of the East China Sea. In the study area, modern and Pleistocene terrigenous sediments and bioclastic accumulations dominate, but biochemi...

Embree Peter, 1986: Resolution and rules of thumb. Geophysics: , Pages 872. 1986.

Singh, Rk, 1987: Residence time distribution in aseptic processing. Principles of aseptic processing and packaging edited by Philip E Nelson James V Chambers Judy H Rodriguez contributors Dane T Howard et al: 7

Neeley, W.E.; Tyson, M.; O'Classen, K.; Gruber, M., 1987: Artifactually elevated BUN values on the Boehringer Mannheim-Hitachi 737 and 705 automated chemistry analysers and the development of a kinetic BUN method. Journal of Automatic Chemistry 9(1): 41-43

Campos, C.A., 1950: Degenerative modifications in diabetes. Revista de la Asociacion Medica Argentina 64(689-690): 522-526

Carino, A.; Graziosi, L.; D'Amore, C.; Cipriani, S.; Marchianò, S.; Marino, E.; Zampella, A.; Rende, M.; Mosci, P.; Distrutti, E.; Donini, A.; Fiorucci, S., 2016: The bile acid receptor GPBAR1 (TGR5) is expressed in human gastric cancers and promotes epithelial-mesenchymal transition in gastric cancer cell lines. NlmCategory="UNASSIGNED">GPBAR1 (also known as TGR5) is a bile acid activated receptor expressed in several adenocarcinomas and its activation by secondary bile acids increases intestinal cell proliferation. Here, we have examined the...

Wiederhold, N.P.; Najvar, L.K.; Bocanegra, R.; Molina, D.; Olivo, M.; Graybill, J.R., 2007: In vivo efficacy of anidulafungin and caspofungin against Candida glabrata and association with in vitro potency in the presence of sera. In vitro studies have demonstrated that anidulafungin has greater potency than caspofungin against Candida glabrata. However, data from in vivo studies demonstrating that it has superior efficacy are lacking. The objective of this study was to co...

Gordon, Li, 1982: Processing of food products in roasting-baking chambers of new type Production of bakeries.1. Izvestiia vysshikh uchebnykh zavedenii pishchevaia tekhnologiia: ) 17-20

Anonymous, 1926: London Hospital Medical College: Prize Distribution. British Medical Journal 2(3418): 87-89