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Development of a sensitive and rapid UPLC-MS/MS method for the determination of koumine in rat plasma: application to a pharmacokinetic study


, : Development of a sensitive and rapid UPLC-MS/MS method for the determination of koumine in rat plasma: application to a pharmacokinetic study. Biomedical Chromatography 27(6): 736-740

A rapid, selective and sensitive method using UPLC-MS/MS was first developed and validated for quantitative analysis of koumine in rat plasma. A one-step protein precipitation with methanol was employed as a sample preparation technique. Plasma samples were separated on an Acquity UPLC BEH C18 column (50 × 2.1 mm, i.d. 1.7 µm) with a gradient mobile phase consisting of methanol with 0.1% (v/v) formic acid and water containing 0.1% (v/v) formic acid at a flow rate of 0.3 mL/min. Detection and quantification were performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring mode via positive eletrospray ionization. Good linearity (r > 0.9997) was achieved using weighted (1/x(2) ) least squares linear regression over a concentration range of 0.025-15 µg/mL with a lower limit of quantification of 0.025 µg/mL for koumine. The intra- and inter- precisions (relative standard deviation) of the assay at all three quality control samples were 5.6-14.1% with an accuracy (relative error) of 5.0-14.0%, which meets the requirements of the US Food and Drug Administration guidance. This developed method was successfully applied to an in vivo pharmacokinetic study in rats after a single intravenous dose of 20 mg/kg koumine.

US$19.90

PMID: 23225577

DOI: 10.1002/bmc.2852


Other references

Dzhalalov A.D.; Arustamyan T.A.; Arustamyan A.T.; Kitainik R.N.; Ruzanova L.S.; Dan'shina N.A.; Belikova T.V.; L.A.B.; Tishkov S.S., 1979: Secretory and motor evacuator function of the stomach in workers of various shops of the samarkand uzbek ssr ussr super phosphate factory suffering from chronic gastritis. Meditsinskii Zhurnal Uzbekistana (1): 64-67

Edwards M.D.; Webb John A., 2004: Groundwater salinity distribution in the Tertiary sand aquifer of the Mount William Creek catchment, upper Wimmera region, western Victoria. Abstracts Geological Society of Australia: ages 21. 2004.

Gayda, M.; Bosquet, L.; Paillard, Fçois.; Garzon, M.; Sosner, P.; Juneau, M.; Bélanger, M.; Nigam, A., 2012: Effects of sauna alone versus postexercise sauna baths on short-term heart rate variability in patients with untreated hypertension. We measured the effects of sauna bathing alone or a 30-minute exercise session followed by sauna bathing on short-term heart rate variability (HRV) in subjects with untreated hypertension. Ten patients with untreated hypertension (age 59 ± 10 yea...

Inoue H.; Konishi Y.; Takeda Y.; Cihak A., 1981: Role of putrescine in modulation of dna synthesis in regenerating liver of rats pre treated with 5 aza cytidine. The relationship between polyamine metabolism and DNA synthesis in regenerating liver was investigated using normal and 5-azacytidine-treated rats. The increase of DNA synthesis in regenerating rat liver was completely inhibited by administration...

Tehrani, F.T.; Roum, J.H., 2008: Flex: a new computerized system for mechanical ventilation. To describe and evaluate a new weaning and decision support system for mechanical ventilation. FLEX is a computerized weaning and decision support system for mechanical ventilation that unlike previous rule-based systems derives many of its rules...

Clarke, C.A., 1964: The Contribution Of Genetics. Journal of the College of General Practitioners 8 Suppl 2: Suppl 2:16-23

Pietschmann, P.; Azizi-Semrad, U.; Pils, K.; Fahrleitner-Pammer, A.; Resch, H.; Dobnig, H., 2011: Pharmacologic undertreatment of osteoporosis in Austrian nursing homes and senior's residences. Osteoporosis is a classical age-related disease. Although significant progress in treatment has been achieved and antifracture efficacy proven over the past years undertreatment is still a general problem. There are only few published data availab...

Dixon Hardy D.W.; Dwomoh M., 2000: Arresting underground tunnel explosions; a computational fluid dynamics study. Traditional methods of arresting underground mine explosions have employed water and/or stone-dust barriers. The first barriers used in underground coal mines operated passively, using the blast wave as the mechanism for discharging the inert mate...

Peters, G.; Von Eiff, C.; Herrmann, M., 1995: The changing pattern of coagulase-negative staphylococci as infectious pathogens. Current Opinion in Infectious Diseases 8(SUPPL 1): S12-S19

Astort, F.; Repetto, E.M.; Martínez Calejman, C.; Cipelli, J.M.; Sánchez, R.; D.G.uccio, J.M.; Mercau, M.; Pignataro, O.P.; Arias, P.; Cymeryng, C.B., 2009: High glucose-induced changes in steroid production in adrenal cells. Increased activity of the hypothalamic-pituitary-adrenal (HPA) axis, resulting in enhanced adrenocorticotropin (ACTH) and serum glucocorticoid levels, has been described in patients with diabetes mellitus and in animal models of this disease; howe...