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A novel application of Cicer ?-galactosidase in reduction of raffinose family oligosaccharides in soybean flour

, : A novel application of Cicer ?-galactosidase in reduction of raffinose family oligosaccharides in soybean flour. Journal of Plant Biochemistry and Biotechnology 22(3): 353-356

Raffinose family oligosaccharide (RFOs) especially raffinose and stachyose are responsible for flatulence during intake of soybean derived products. This gives negative impression on the acceptance of soy-based foods. The effect of soaking, cooking and Cicer ?-galactosidase treatment for reducing stachyose and raffinose content from the soybean flour was investigated. Cicer ?-galactosidase treatment was found to be most effective in comparison to traditional methods, soaking and cooking. Enzymatic treatment for 6 h reduces raffinose and stachyose component of soybean flour by 80.0 % and 85.0 %, respectively. This can be utilized effectively for improving the nutritional quality of soy-based foods on large scale, which could be one of the best alternatives for lactose intolerant population for fulfilling their protein requirement.


DOI: 10.1007/s13562-012-0173-7

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