+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Matters of the heart in bioenergetics: mitochondrial fusion into continuous reticulum is not needed for maximal respiratory activity

, : Matters of the heart in bioenergetics: mitochondrial fusion into continuous reticulum is not needed for maximal respiratory activity. Journal of Bioenergetics and Biomembranes 45(4): 319-331

Mitochondria are dynamic structures for which fusion and fission are well characterized for rapidly dividing cells in culture. Based on these data, it has recently been proposed that high respiratory activity is the result of fusion and formation of mitochondrial reticulum, while fission results in fragmented mitochondria with low respiratory activity. In this work we test the validity of this new hypothesis by analyzing our own experimental data obtained in studies of isolated heart mitochondria, permeabilized cells of cardiac phenotype with different mitochondrial arrangement and dynamics. Additionally, we reviewed published data including electron tomographic investigation of mitochondrial membrane-associated structures in heart cells. Oxygraphic studies show that maximal ADP-dependent respiration rates are equally high both in isolated heart mitochondria and in permeabilized cardiomyocytes. On the contrary, these rates are three times lower in NB HL-1 cells with fused mitochondrial reticulum. Confocal and electron tomographic studies show that there is no mitochondrial reticulum in cardiac cells, known to contain 5,000 10,000 individual, single mitochondria, which are regularly arranged at the level of sarcomeres and are at Z-lines separated from each other by membrane structures, including the T-tubular system in close connection to the sarcoplasmic reticulum. The new structural data in the literature show a principal role for the elaborated T-tubular system in organization of cell metabolism by supplying calcium, oxygen and substrates from the extracellular medium into local domains of the cardiac cells for calcium cycling within Calcium Release Units, associated with respiration and its regulation in Intracellular Energetic Units.


PMID: 23271420

DOI: 10.1007/s10863-012-9494-4

Other references

Monkheang, P.; Chaveerach, A.; Sudmoon, R.; Tanee, T., 2017: Karyotypic features including organizations of the 5S, 45S rDNA loci and telomeres of iScadoxus multiflorus (Amaryllidaceae). <i>Scadoxus multiflorus</i> Martyn, 1795 is an ornamental plant with brilliantly colored flowers. Even though its chromosomes are rather large, there is no karyotype description reported so far. Therefore, conventional and molecular cy...

Rodriguez, E.; Lopez, R.; Paez, A., 2002: 17b-estradiol inhibits the adhesion of leukocytes in TNF-a stimulated human endothelial cells by blocking IL-8 and MCP-1 secretion, but not its transcription. Life Sciences 71(18): 81-93

Schumacher, K.; Schondelmaier, J.; Barzen, E.; Steinrucken, G.; Borchardt, D.; Weber, W.E.; Jung, C.; Salamini, F., 1997: Combining different linkage maps in sugar beet (Beta vulgaris L.) to make one map. Several genetic maps for sugarbeet (Beta vulgaris), from different German research groups, have been published; this paper considers combining them with the aid of common markers. The computer program JOINMAP (versions 1.3 and 2.0) was used for pa...

Magnusson, S.P.; Simonsen, E.B.; Dyhre Poulsen, P.; Aagaard, P.; Mohr, T.; Kjaer, M., 1996: Viscoelastic stress relaxation during static stretch in human skeletal muscle in the absence of EMG activity. The present study sought to investigate the role of EMG activity during passive static stretch. EMG and passive resistance were measured during static stretching of human skeletal muscle in eight neurologically intact control subjects and six spin...

Morimoto, K.; Kawada, A.; Hiruma, M.; Ishibashi, A., 1994: An evaluation of whitening effect of serum clarifiant against UVB-induced delayed tanning. Acta Dermatologica (Kyoto) 89(4): 675-678

Appelmans, M.; D.N.El.J.; Lebas, P., 1951: Physiotherapy of bilateral gliomas of the retina. Bulletin de la Societe Belge D'ophtalmologie 99: 532-551

Gray, V.L.; Yang, C-Ling.; McCombe Waller, S.; Rogers, M.W., 2017: Lateral Perturbation-Induced Stepping: Strategies and Predictors in Persons Poststroke. Falls commonly occur as weight is transferred laterally, and impaired reactive stepping responses are associated with falls after stroke. The purpose of this study was to examine differences in and the determinants of mediolateral (M-L) protective...

Chen, A.P.; Albers, M.J.; Cunningham, C.H.; Kohler, S.J.; Yen, Y-Fen.; Hurd, R.E.; Tropp, J.; Bok, R.; Pauly, J.M.; Nelson, S.J.; Kurhanewicz, J.; Vigneron, D.B., 2007: Hyperpolarized C-13 spectroscopic imaging of the TRAMP mouse at 3T-initial experience. The transgenic adenocarcinoma of mouse prostate (TRAMP) mouse is a well-studied murine model of prostate cancer with histopathology and disease progression that mimic the human disease. To investigate differences in cellular bioenergetics between...

Alexandra Livarda; Marijke van der Veen, 2008: Social access and dispersal of condiments in North-West Europe from the Roman to the medieval period. The introduction and dispersal histories of eight common condiments in North-West Europe are explored: Apium graveolens (celery), Anethum graveolens (dill), Coriandrum sativum (coriander), Brassica nigra (black mustard), Foeniculum vulgare (fennel...

Goto, T.; Enmoto, T.; Akimoto, K., 2009: Diagnosis of an ilio-iliac arteriovenous fistula by multidetector row computed tomography and surgical repair. An 82-year-old man presented with typical signs and symptoms of an arteriovenous fistula (AVF) associated with an aortoiliac aneurysm, such as a pulsatile abdominal mass, continuous bruit, thrill, and high output heart failure. Multidetector row c...