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TACTICS, an Interactive Platform for Customized High Content Bioimaging Analysis

, : TACTICS, an Interactive Platform for Customized High Content Bioimaging Analysis.

We describe a modular MATLAB® Toolbox named TACTICS for time lapse image analysis that meets several requirements not generally offered by currently available software packages: (i) the ability to assess quality of extracted imaging information by directly linking data endpoints to the original position, (ii) massively parallel analysis of each parameter, for flow cytometry-like assessment of possible relationships between parameters within subpopulations of the images, (iii) options for user-control of the tracking such as an interface to restrict the analysis region, (iv) manual correction of automated processes, and (v) user interfaces for post-tracking analysis that is linked to the original images, including options to view cell pedigrees and normalized polarization ratios based on fluorescence ratiometric measurements.


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