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The dynamics of induced pluripotency and its behavior captured in gene network motifs


, : The dynamics of induced pluripotency and its behavior captured in gene network motifs.

The flexibility of cellular identity is clearly demonstrated by the possibility to reprogram fully differentiated somatic cells to induced pluripotent stem (iPS) cells through forced expression of a set of transcription factors. The generation of iPS cells is of great interest as they provide a tremendous potential for regenerative medicine and an attractive platform to investigate pluripotency. Despite having gathered much attention, the molecular details and responsible gene regulatory networks during the reprogramming process are largely unresolved. In this review we analyze the sequence and dynamics of reprogramming to construct a time-line of the molecular events taking place during induced pluripotency. We use this time-line as a road map to explore the distinct phases of the reprogramming process and to suggest gene network motifs that are able to describe its systems behavior. We conclude that the gene networks involved in reprogramming comprise several feedforward loops combined with autoregulatory behavior and one or more AND gate motifs that can explain the observed dynamics of induced pluripotency. Our proposed timeline and derived gene network motif behavior could serve as a tool to understand the systems behaviour of reprogramming and identify key transitions and/or transcription factors that influence somatic cell reprogramming. Such a systems biology strategy could provide ways to define and explore the use of additional regulatory factors acting at defined gene network motifs to potentially overcome the current challenges of inefficient, slow and partial somatic cell reprogramming and hence set ground of using iPS cells for clinical and therapeutic use.

US$19.90

DOI: 10.1002/stem.1340


Other references

Kaffenberger D.A.; Heinz E.R.; Oakes J.W.; Boyko O., 1992: Meningocele manque radiologic findings with clinical correlation. Purpose: To determine whether meningocele manque can be detected by neuroimaging techniques in dysraphic patients. Methods: We reviewed the records and imaging studies of 16 patients with surgically proved meningocele manque seen at our institutio...

Adamczyk, A., 2009: Understanding the effects of personal and school religiosity on the decision to abort a premarital pregnancy. Although much research has examined the relationship between religion and abortion attitudes, few studies have examined whether religion influences abortion behavior. This study looks at whether individual and school religiosity influence reported...

Fiore J.R.; Angarano G.; Fico C.; Fracasso C.; Carbonara S.; Pastore G., 1990: Technical notes on hiv isolation from peripheral blood lymphocytes use of pha. As a part of ongoing study about the role that different technical factors may play in influencing the sensibility of HIV isolation procedures, the authors have evaluated the effects of PHA stimulation of infected cells on HIV replication in cell...

Snapper, S.B.; Melton, R.E.; Mustafa, S.; Kieser, T.; Jacobs, W.R., 1990: Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Recent development of vectors and methodologies to introduce recombinant DNA into members of the genus Mycobacterium has provided new approaches for investigating these important bacteria. While most pathogenic mycobacteria are slow-growing, Mycob...

Kovacikova Z.; Reichrtova E., 1986: Animal alveolar macrophages as indicators of industrial dust pollution. Chamber and field dust pollution monitoring on laboratory rats and rabbits was used for evaluating the effect of magnesite and cement industrial dust on alveolar ma rophages. Animals were exposed for 6 months to magnesite emissions and for 20 mont...

Kleitman, Nathaniel, 1927: Influence of starvation on the rate of secretion of saliva elicited by pilocarpine, and its bearing on conditioned salivation. Dogs with salivary fistulae were given 0.5 mgm. pilocarpine sulphate per kgm. subcutaneously every day. No conditioned salivary reflex was established, although daily injections of morphine do result in the establishment of a conditioned reflex. I...

Shaw, M.G.; Kocan, A.A., 1980: Helminth fauna of waterfowl in central Oklahoma. Free-ranging waterfowl wintering in and migrating through central Oklahoma [USA] were collected and examined for intestinal helminths. Seventy-one ducks, including mallards (A. platyrhynchos), American widgeons (A. americana), blue-winged teal (A....

Ding LianSheng; Zhang WeiGuo; Han TianHu, 1998: The relationship between population size of Myospalax baileyi and its reproductive characteristics. The relationship between the population fluctuation of Myospalax baileyi and its reproductive characteristics such as sex ratio, rate of pregnancy, litter size and reproductive intensity were studied for 3 years in 3 populations. It was shown that...

Yarbrough, John A., 1936: Regeneration in the foliage leaf of Sedum. At the time of leaf detachment no preformed rudiments or foliar embryo is present but such rudiments are readily formed by a pad of callus tissue arising from the short petiole. The roots arise endogenously and the shoot from superficial tissue of...

Bacanlı, M.; Başaran, A.Ahmet.; Başaran, Nşen., 2016: The antioxidant and antigenotoxic properties of citrus phenolics limonene and naringin. Phenolic compounds not only contribute to the sensory qualities of fruits and vegetables but also exhibit several health protective properties. Limonene and naringin are the most popular phenolics found in Citrus plants. In this study, we investig...