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Differential expression of two isolates of beak and feather disease virus capsid protein in Escherichia coli

, : Differential expression of two isolates of beak and feather disease virus capsid protein in Escherichia coli. Journal of Virological Methods 189(1): 118-124

Expression of recombinant beak and feather disease virus (BFDV) capsid-associated protein (Cap) has relied on inefficient techniques that typically produce low yields or use specialized expression systems, which greatly increase the cost and expertise required for mass production. An Escherichia coli system was used to express recombinant BFDV Cap derived from two isolates of BFDV, from a Long-billed Corella (Cacatua tenuirostris) and an Orange-bellied parrot (OBP; Neophema chrysogaster). Purification by affinity and size exclusion chromatography was optimized through an iterative process involving screening and modification of buffer constituents and pH. A buffer containing glycerol, ?-mercaptoethanol, Triton X-100, and a high concentration of NaCl at pH 8 was used to increase solubility of the protein. The final concentration of the corella-isolated BFDV protein was fifteen- to twenty-fold greater than that produced in previous publications using E. coli expression systems. Immunoassays were used to confirm the specific antigenicity of recombinant Cap, verifying its validity for use in continued experimentation as a potential vaccine, a reagent in diagnostic assays, and as a concentrated sample for biological discoveries. Expression of two isolates of BFDV capsid protein in E. coli Optimization of purification buffers improve recombinant protein yield Amino acid sequence affects recombinant protein solubility Produced a higher concentration of BFDV capsid protein than previously reported.


PMID: 23403150

DOI: 10.1016/j.jviromet.2013.01.020

Other references

Barnes, Nr, 1992: Children need guidance. Information sheet Mississippi State University Cooperative Extension Service: 92 (1459)

Kabakov, V.I., 1955: The fattening of pigs from a commercial cross between Bulgarian White sows and Cornwall boars. To determine the fattening ability of commercial crosses between Cornwall boars and Bulgarian White sows, comparative investigations were made on 2 groups of 16 piglings (8 and 8), one composed of purebred Whites and the other of crossbreds. The a...

Sengbusch, R.Von., 1960: The way to the Max Planck Institute of Crop Breeding. The various stages involved in developing cultivated forms from wild and in improving cultivated material are described with reference to work conducted at the above institute, and in particular to the author's own work since 1924.

Langberg, J.J.; Underwood, T.; Gallagher, M.; Labhasetwar, V.; Levy, R., 1993: The effects of ibutilide applied to the epicardium on defibrillation threshold. Journal of the American College of Cardiology 21(2 SUPPL A): 244A

Mendoza, C.E.; Shields, J.B.; Phillips, W.E., 1971: Distribution of carboxylesterase activities in different tissues of albino rats. 1.1. Esterase activities on indophenyl acetate were determined in extracts of livers, kidneys, brain, thigh muscles and testes of albino rats.2.2. Livers and kidneys had higher esterase activity than brain, muscles or testes.3.3. No significant lo...

Liu, D.; Hostetler, C.; Miller, I.; Cook, A.; Hair, J., 2012: System analysis of a tilted field-widened Michelson interferometer for high spectral resolution lidar. High spectral resolution lidars (HSRLs) have shown great value in aircraft aerosol remote sensing application and are planned for future satellite missions. A compact, robust, quasi-monolithic tilted field-widened Michelson interferometer is being...

Breger, R.K.; Franklin, R.B.; Lech, J.J., 1981: Metabolism of 2-methylnaphthalene to isomeric dihydrodiols by hepatic microsomes of rat and rainbow trout. The metabolism of 2-methylnaphthalene in rats (in vivo and in vitro) and rainbow trout (in vitro) has been investigated. Three isomeric dihydrodiols were formed by microsomal preparations and these were isolated and identified by high-pressure liq...

Starrett, J.; Hayashi, C.Y.; Derkarabetian, S.; Hedin, M., 2017: Cryptic elevational zonation in trapdoor spiders (Araneae, Antrodiaetidae, Aliatypus janus complex) from the California southern Sierra Nevada. The relative roles of ecological niche conservatism versus niche divergence in promoting montane speciation remains an important topic in biogeography. Here, our aim was to test whether lineage diversification in a species complex of trapdoor spid...

Demidova, I.A.; Savchenko, V.G.; Ol'shanskaia, I.V.; Poreshina, L.P.; Kut'ina, R.M.; Surin, V.L.; Misiurin, A.V.; Domracheva, E.V.; Parovichnikova, E.N., 2003: Allogenic bone marrow transplantation after reduced intensity conditioning regimens in therapy of patients with hemoblastoses. To investigate effectiveness of allogenic transplantation of the bone marrow (TBM) in the treatment of hemoblastosis patients from a high risk group, the course of donor bone marrow retention, tolerance and antitumor activity of this therapy. 11 p...

Munn, H.E., 1975: "Developing and improving your communication skills". Hospital Topics 53(6): 40-43