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In vitro effects of aldehydes present in tobacco smoke on gene expression in human lung alveolar epithelial cells

, : In vitro effects of aldehydes present in tobacco smoke on gene expression in human lung alveolar epithelial cells. Toxicology in Vitro 27(3): 1072-1081

Tobacco smoke consists of thousands of harmful components. A major class of chemicals found in tobacco smoke is formed by aldehydes, in particular formaldehyde, acetaldehyde and acrolein. The present study investigates the gene expression changes in human lung alveolar epithelial cells upon exposure to formaldehyde, acrolein and acetaldehyde at sub-cytotoxic levels. We exposed A549 cells in vitro to aldehydes and non-aldehyde chemicals (nicotine, hydroquinone and 2,5-dimethylfuran) present in tobacco smoke and used microarrays to obtain a global view of the transcriptomic responses. We compared responses of the individual aldehydes with that of the non-aldehydes. We also studied the response of the aldehydes when present in a mixture at relative concentrations as present in cigarette smoke. Formaldehyde gave the strongest response; a total of 66 genes were more than 1.5-fold differentially expressed mostly involved in apoptosis and DNA damage related processes, followed by acetaldehyde (57 genes), hydroquinone (55 genes) and nicotine (8 genes). For acrolein and the mixture only one gene was upregulated involved in oxidative stress. No gene expression effect was found for exposure to 2,5-dimethylfuran. Overall, aldehyde responses are primarily indicative for genotoxicity and oxidative stress. These two toxicity mechanisms are linked to respiratory diseases such as cancer and COPD, respectively. The present findings could be important in providing further understanding of the role of aldehydes emitted from cigarette smoke in the onset of pulmonary diseases. Aldehydes induce gene expression changes at sub-cytotoxic exposures. The gene expression profiles are indicative of genotoxicity and oxidative stress. Response to aldehyde mixture comprises signatures of the individual components.


PMID: 23416264

DOI: 10.1016/j.tiv.2013.02.003

Other references

Beloiartsev, F.F.; Meĭtina, R.A.; Khapĭ, K.Kh.; Chernikov, V.S.; Kurochkin, V.M., 1979: Gas exchange and oxygen transport in blood during fluid ventilation of the lungs. Patologicheskaia Fiziologiia i Eksperimental'naia Terapiia: 8-11

Davenport, D.; Jensen, M.B., 1971: Intrepid analysis of female scientists. Science 171(3971): 521-522

Seneca, H., 1959: Management of Aerobacter and Escherichia in urinary tract infections. Journal of Urology 81(2): 324-329

Bauch D.; Erlenkeuser H.; Stanovoy V.; Simstich J.; Spielhagen R.F., 2003: Freshwater distribution and brine waters in the southern Kara Sea in summer 1999 as depicted by delta (super 18) O results. Proceedings in Marine Science 6: 73-90

Grinnell, J., 1918: The subspecies of the Mountain Chickadee. Berkeley University of California Publ Zoology, 17 505-515

Mole, S.E.; Mulligan, L.M.; Healey, C.S.; Ponder, B.A.; Tunnacliffe, A., 1993: Localisation of the gene for multiple endocrine neoplasia type 2A to a 480 kb region in chromosome band 10q11.2. A YAC contig has been constructed spanning 1.1 Mb of human chromosome band 10q11.2 which encompasses three markers (D10S141, RET, D10S94) closely linked to the gene for MEN 2A. This physical linkage group is ordered cen-D10S141-RET-D10S94-qter, an...

Yu, X.; Ye, Z.; Yang, W.; Deng, R., 2004: Efficacy of local injection of bacillus calmette-guerin polysaccharide nucleic acid following CO2 laser resection on condyloma acuminatum. To observe the efficacy of local injection of bacillus calmette-guerin polysaccharide-nuclear acid (BCG-PSN) following CO2 laser resection on condyloma acuminatum (CA). One hundred and six patients with CA were randomized into BCG-PSN group (60 ca...

Popov, A.; Popov, B., 1980: Morphological studies of the small intestine in experimental operations to isolate segments from the digestive tract. Changes in the small intestine of white rats, developing within fifteen days of experimental operation for the isolation of a segment outside the digestive tract, are investigated using light and fluorescent microscopy. The isolated segment with i...

Vibert R., 1968: Symposium of the commission europeenne consultative pour les peches dans les eaux interieures consultative european commission for fishing in inland waters on applications of electricity to biology and to the management of continental fishing section on electric screens and guides belgrade yugoslavia may 1966. Vibert, R (Editor) Applications De L'electricite A La Biologie Et A L'amenagement Des Peches Continentales (Application Of Electricity in Interior Waters) 276p Illus Institut National De La Recherche Agronomique: 149 Rue De Grenelle, Paris, France 191-246

Mehta Sunil; Pani Eric A.; Washington Paul A., 1996: Correlation of water levels with rainfall and among well populations as a tool for distinguishing groundwater sources. Abstracts with Programs - Geological Society of America 28(2): 38