+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

C3 exoenzyme lacks effects on peripheral axon regeneration in vivo

, : C3 exoenzyme lacks effects on peripheral axon regeneration in vivo. Journal of the Peripheral Nervous System 18(1): 30-36

Peripheral nerve injury triggers the activation of the small GTPase RhoA in spinal motor and peripheral sensory neurons. C3 transferase, an exoenzyme produced by Clostridium botulinum that inactivates RhoA by ADP-ribosylation, has been successfully applied in central nervous system (CNS) lesion models to facilitate regeneration functionally and morphologically. Until now it has not been demonstrated if C3bot exerts positive effects on peripheral axon regeneration as well. In organotypic spinal cord preparations, C3bot reduced axonal growth of motoneurons, while no effect on sensory axon outgrowth from dorsal root ganglia (DRG) explants was observed. Enzymatically inactive C3E174Q was ineffective in both culture models. Spinal cord slices exhibited a significant increase in microglia/macrophages after treatment with C3bot suggesting an inflammatory component in the inhibition of axon growth. C3bot or C3E174Q were then applied into conduits implanted after transection of the sciatic nerve in rats. Functional evaluation by electrophysiology, nociception, and walking track tests did not show any significant difference between groups with active or mutant C3E174Q . Transmission electron microscopy of the regenerated nerves revealed no significant differences in the number of myelinated and unmyelinated axons 6 weeks after surgery. Compared to the CNS, the functional significance of RhoA may be limited during nerve regeneration in a growth-promoting environment.


PMID: 23521641

DOI: 10.1111/jns5.12004

Other references

Harkrider, JR.; Hall, IM., 1979: The effect of an entomopoxvirus on larval populations of an undescribed midge species in the Chironomus decorus complex under laboratory conditions. Larvae of an undescribed species in the complex of Chironomus decorus Joh. reared at densities of 0.3-8.4 larvae/cm2 were inoculated with an entomopoxvirus originally found in a field population in southern California of the same midge, which is a...

Marshak, R.H.; Eliasoph, J., 1958: Radiological notes: case No. 60. Journal of the Mount Sinai Hospital, New York 25(6): 600-603

Brandt, E.N., 1981: Funding cuts and the nation's health--an interview with the government's top doctor. Medical World News 22(16): 34-5, 37-8, 40

Rossato, N.Elena., 2014: Newborn sleep positioners and sudden infant death syndrome risk. The rate of sudden infant death decreased after the publication of the first guidelines regarding infant sleep position and safe environment in 1992. From 2005 onwards, infant deaths by suffocation, choking or entrapment have increased. Some of th...

Webb, D.; Jamison, T.F., 2012: Diisobutylaluminum hydride reductions revitalized: a fast, robust, and selective continuous flow system for aldehyde synthesis. A continuous flow system for the multiparameter (flow rate, temperature, residence time, stoichiometry) optimization of the DIBALH reduction of esters to aldehydes is described. Incorporating an in-line quench (MeOH), these transformations are gen...

D'Antonio, Costantino., 1996: The dragonflies from Calabria Southern Italy Odonata Gli Odonati della Calabria Odonata. Bollettino della Societa Entomologica Italiana. Gennaio-Aprile; 1281: 3-16

Hughes, C.; Drew, P.G.; Friedman, R.J., 1995: Magnetic resonance imaging: a bright future despite health care's cost-conscious environment. Hospital Technology Series 14(14): 1-43

Van Echten Deckert, G.; Zschoche, A.; Bar, T.; Schmidt, R.R.; Raths, A.; Heinemann, T.; Sandoff, K., 1997: CIS-4-methylsphingosine-1-phosphate as a potential metabolically stable nimetic of sphingosine-1-phosphate activity. Journal of Neurochemistry 69(SUPPL ): S18

Milly, Pcd, 1992: Potential evaporation and soil moisture in general circulation models. The parameterization of continental evaporation in many atmospheric general circulation models (GCMs) used for simulation of climate is demonstrably inconsistent with the empirical work upon which the parameterization is based. In the turbulent tr...

Alder, S., 2002: Are ALF premium hikes fair? Increases may be based more on fear than fact. Contemporary Longterm Care 25(1): 16-17