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TGF-β1 induces a nucleus pulposus-like phenotype in Notch 1 knockdown rabbit bone marrow mesenchymal stem cells


, : TGF-β1 induces a nucleus pulposus-like phenotype in Notch 1 knockdown rabbit bone marrow mesenchymal stem cells. Cell Biology International 37(8): 820-825

We have investigated the effects of Notch1 knockdown and treatment with TGF-β1 on the regulation of the directional differentiation of mesenchymal stem cells (MSCs). MSCs were isolated from the femur bone of New Zealand rabbits and purified by using discontinuous gradient density centrifugation. Notch1 siRNAs were designed, synthesised and transiently transfected into these MSCs, and treated with TGF-β1. The MSCs were examined for morphology, stained with toluidine blue for proteoglycan analysis and gene and protein expression were measured with qRT-PCR and Western blotting respectively. They had an ellipse or fusiform shape and gathered in nests or swirls after being cultured for 7 days. Notch1 expression was knocked down with Notch1 siRNA (the silence rate was 47%; P < 0.001). After knockdown and TGF-β1 treatment, MSCs expressed more proteoglycan (P < 0.01), and higher levels of collagen II mRNA and protein than control cells (P < 0.001). Thus knockdown of Notch1 expression in MSCs may be useful in the treatment of intervertebral disc degeneration.

US$19.90

PMID: 23554118

DOI: 10.1002/cbin.10109


Other references

Johnson G.L., 1989: Chimeras that alter the amino or carboxyl terminus of the alpha subunit of g s a cyclic amp synthesis. Journal of Cellular Biochemistry Supplement (13 PART E): 252

Paoloni Giacobino, A.; Malafosse, A.; Buresi, C., 1999: Identification and analysis of new sequence variants in human tryptophan hydroxylase gene. European Journal of Human Genetics 7(SUPPL 1): 146, July

Murthy, S.V.K.; Iyengar, B.R.V., 1992: Efficiency of N absorption, rate of N uptake and utilisation in Citrus and Poncirus rootstocks as influenced by their root morphology. Nitrogen absorption and translocation were studied in 6 seedling rootstocks and seedlings of the mandarin cultivar Coorg, grown for a year in pots containing a sandy clay loam soil. The rootstocks comprised rough lemon, Rangpur lime [Citrus limoni...

Nygren, A.; Thorén, A.; Ricksten, S-Erik., 2003: Effects of norepinephrine alone and norepinephrine plus dopamine on human intestinal mucosal perfusion. To evaluate the effect of norepinephrine alone and norepinephrine combined with dopamine on jejunal mucosal perfusion, gastric-arterial pCO(2) gradient, and global splanchnic oxygen demand-supply relationship after cardiac surgery. A prospective i...

Dalgleish, S.; Finlayson, D., 2013: Caution is needed in interpreting results. Bmj 345: E7144-E7144

Perdigão, C.; Andrade, A.; Ribeiro, C., 1989: Intraventricular thrombi in acute myocardial infarct. Clinicoanatomic study carried out in 193 consecutive cases. To determine the incidence of intraventricular thrombi in patients who died of acute myocardial infarction, as well as to define the clinico-anatomical parameters of the population with thrombi. CONCEPT OF THE STUDY: To apply a clinical as well as...

Vardjan M., 1975: Regulation of dormancy bud break and germination. Adamic, F (Ed ) Spominski Zbornik Biotehniske Fakultete Univerze V Ljubljani Ob Stoletnici Rojstva Prof Dr Frana Jesenko (1875-1932) (Commemorative Collection Of The Biotechnical Faculty Of The University Of Ljubljana on The Hundredth Anniversary Of The Birth Of Prof Dr Fran Jesenko (1875-1932)) 98p Illus Central Library Of The Biotechnical Faculty University Of Ljubljana: Ljubljana, Yugoslavia 25-32

Clauwaert, K.M.; Van Bocxlaer, J.F.; D.L.tter, E.A.; Van Calenbergh, S.; Lambert, W.E.; D.L.enheer, A.P., 2000: Determination of the designer drugs 3, 4-methylenedioxymethamphetamine, 3,4-methylenedioxyethylamphetamine, and 3,4-methylenedioxyamphetamine with HPLC and fluorescence detection in whole blood, serum, vitreous humor, and urine. The popular designer drugs 3, 4-methylenedioxymethamphetamine (MDMA) and 3, 4-methylenedioxyethylamphetamine (MDEA) can be determined in serum, whole blood, and urine, but also in vitreous humor. The latter matrix is interesting when dealing with...

Passechnik, V.I., 1978: Thermal fluctuations in membranes and their influence in mechano reception. In order to estimate thermal fluctuations in [human] erythrocyte and mechanoreceptor membranes the transverse fluctuations of plane and spherical bilayer membranes and the fluctuations of the surface of a part of such a membrane, possessing a disc...

Kavukcu, S.; Turkmen, M.-Atilla; Soylu, A., 2001: Could the effective mechanisms of retinoids on nephrogenesis be also operative on the amelioration of injury in acquired renal lesions?. Pediatric Nephrology 16(8): 689-690