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Induced degradation of Tat by nucleocapsid (NC) via the proteasome pathway and its effect on HIV transcription

, : Induced degradation of Tat by nucleocapsid (NC) via the proteasome pathway and its effect on HIV transcription. Viruses 5(4): 1143-1152

Human Immunodeficiency Virus type 1 (HIV-1) is a retrovirus that causes acquired immunodeficiency syndrome (AIDS). HIV-1 Tat protein upregulates transcriptional transactivation. The nucleocapsid protein NC of HIV-1 is a component of virion and plays a key role in genome packaging. Herein, we have demonstrated the interaction between NC and Tat by means of a yeast two-hybrid assay, GST pull-down analysis, co-immunoprecipitation and subcellular colocalization analysis. We observed that the level of Tat was significantly reduced in the presence of NC. But NC did not affect mRNA expression level of Tat. The level of Tat in the presence of NC was increased by treating cells with a proteasome inhibitor, MG132. The ubiquitination state of Tat was not seen to increase in the presence of NC, suggesting the proteasomal degradation was independent of ubiquitination. Lowered level of Tat in the presence of NC led to a decrease in Tat-mediated transcriptional transactivation.


PMID: 23611845

DOI: 10.3390/v5041143

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Sadeh, B.; Pitcher, D.; Brandman, T.; Eisen, A.; Thaler, A.; Yovel, G., 2012: Stimulation of category-selective brain areas modulates ERP to their preferred categories. Neural selectivity to specific object categories has been demonstrated in extrastriate cortex with both functional MRI [1-3] and event-related potential (ERP) [4, 5]. Here we tested for a causal relationship between the activation of category-sele...

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Mackie C.D., 1987: Conceptual design criteria for seepage recharge structures. Australian Water Resources Council Conference Series 13: 451-459

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Tinne, J.E., 1971: Studies of the geographical spread of drug-resistant tubercle bacilli in a city. Journal of Medical Microbiology 4(2): P3-P3

Shammas, N.W.; Chammas, M.Z.; Robken, J.; Geiss, D., 2017: Percutaneous Closure of the Aorto-Ostial Origin of a Coronary Artery Saphenous Bypass Graft with a Large Pseudoaneurysm Using the AMPLATZER Muscular Ventricular Septal Defect Occluder. We report the case of a 76-year-old male patient with a history of coronary artery bypass graft surgery presented with a large pseudoaneurysm emerging from a previously occluded saphenous bypass graft (SVG). A largely contained hematoma is seen in...

Cohen, Y.; Flaishon, R.; Lichtenberg, L., 2001: Opening anesthesiologist-proof vials. Anesthesiology 95(3): 814-814

Efremov, V.V.; Sakaeva, E.A., 1978: Supply and requirements of vitamin e in adolescent athletes. The serum vitamin E content of adolescent sportsmen aged 13-16 was studied. The observations were carried out under conditions of sporting assemblies. The adolescents were divided into groups, which received 25, 50 and 100 mg vitamin E in the form...