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Bead assembly magnetorotation as a signal transduction method for protein detection

, : Bead assembly magnetorotation as a signal transduction method for protein detection. Biosensors & Bioelectronics 48: 26-32

This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1?m magnetic beads into a bead assembly, formed at the bottom of a 1?L hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field.


PMID: 23639345

DOI: 10.1016/j.bios.2013.03.073

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