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Bead assembly magnetorotation as a signal transduction method for protein detection


, : Bead assembly magnetorotation as a signal transduction method for protein detection. Biosensors & Bioelectronics 48: 26-32

This paper demonstrates a proof-of-principle for a new signal transduction method for protein detection called Bead Assembly Magnetorotation (BAM). BAM is based on using the target protein to mediate the formation of aptamer-coated 1?m magnetic beads into a bead assembly, formed at the bottom of a 1?L hanging droplet. The size, shape and fractal dimension of this bead assembly all depend on the protein concentration. The protein concentration can be measured in two ways: by magnetorotation, in which the rotational period of the assembly correlates with the protein concentration, or by fractal analysis. Additionally, a microscope-free magnetorotation detection method is introduced, based on a simple laser apparatus built from standard laboratory components. In this paper, we chose to focus on the protein thrombin, a popular choice for proof-of-principle work in this field.

US$19.90

PMID: 23639345

DOI: 10.1016/j.bios.2013.03.073


Other references

Rosinska, M.; Janiec, J.; Niedźwiedzka-Stadnik, M., 2014: Increase of new HIV diagnoses among men who have sex with men in Poland, 2000 to 2011. Re-emergence of the human immunodeficiency virus (HIV) epidemic among men who have sex with men (MSM) has been observed in countries of western Europe, North America and Australia since the mid-1990s. We aimed to describe the trends in notificatio...

Pritsch G., 1971: Honey bees role in pollination of broad bean vicia faba. Int Fed Beekeep Assoc Xxiiird International Apicultural Congress Moscow, Ussr Aug 27-Sept 2, 677p Illus Map Apimondia Publishing House: Bucharest, Romania 507-508

Sharp, P.A., 1992: TATA-binding protein is a classless factor. Cell 68(5): 819-821

Mason, D.K.; Watkins, K.L.; Luk, C.M., 1989: Haematological changes in two thoroughbred horses in training with confirmed equine herpesvirus 1 infections. An outbreak of respiratory disease among Thoroughbred horses in training in Hong Kong was caused by equine herpesvirus 1 (EHV-1) subtype 1 (abortion strain). Two of the horses affected by EHV-1 were serially blood sampled over a period of several...

Murray J.S.; Madri J.; Tite J.; Carding S.R.; Bottomly K., 1989: Mhc control of cd4 positive t cell subset activation. The present results demonstrate that CD4+ T cells activated in the primary in vivo response to antigen produce distinct pattern of cytokines depending upon the MHC class II haplotype of the responding mice. I-As mice were found to selectively acti...

McNichol Ann P.; Gagnon Alan R.; Jones Glenn A.; Schneider Robert J.; Von Reden Karl, 1991: The optimization of target production for accelerator mass spectrometry. Radiocarbon 33(2): 224

van der Westhuizen, R.; Ajam, M.; D.C.ning, P.; Beens, J.; de Villiers, Aé.; Sandra, P., 2011: Comprehensive two-dimensional gas chromatography for the analysis of synthetic and crude-derived jet fuels. Fully synthetic jet fuel (FSJF) produced via Fischer-Tropsch (FT) technology was recently approved by the international aviation fuel authorities. To receive approval, comparison of FSJF and crude-derived fuel and blends on their qualitative and q...

Beentjes, B.I., 1972: The cochlear aqueduct and the pressure of cerebrospinal and endolabyrinthine fluids. Acta Oto-Laryngologica 73(2): 112-120

Hacker, H.H. .; Schreier, H.-Peter ., 2002: Insect-fauna of Yemen. Part 3. Esperiana, 9: 55-206, 481-510

Li, C.; Levitz, M.; Hubbard, G.B.; Jenkins, S.L.; Han, V.; Ferry, R.J.; McDonald, T.J.; Nathanielsz, P.W.; Schlabritz-Loutsevitch, N.E., 2007: The IGF axis in baboon pregnancy: placental and systemic responses to feeding 70% global ad libitum diet. Information on the influence of poor maternal nutrition on the regulation of responses to pregnancy, placental and fetal growth and development is critical to a better understanding of pregnancy physiology and pathophysiology. We determined normal...