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Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: reduction of electron-optical effects of charging

, : Cryomicroscopy of radiation sensitive specimens on unmodified graphene sheets: reduction of electron-optical effects of charging. Journal of Structural Biology 183(3): 531-536

Images of radiation-sensitive specimens obtained by electron microscopy suffer a reduction in quality beyond that expected from radiation damage alone due to electron beam-induced charging or movement of the specimen. For biological specimens, charging and movement are most severe when they are suspended in an insulating layer of vitreous ice, which is otherwise optimal for preserving hydrated specimens in a near native state. We image biological specimens, including a single particle protein complex and a lipid-enveloped virus in thin, vitreous ice films over suspended sheets of unmodified graphene. We show that in such preparations, the charging of ice, as assessed by electron-optical perturbation of the imaging beam, is eliminated. We also use the same specimen supports to record high resolution images at liquid nitrogen temperature of monolayer paraffin crystals grown over graphene.


PMID: 23664842

DOI: 10.1016/j.jsb.2013.04.014

Other references

Taguchi, E., 1983: New Middle Miocene Mollusca from the Katsuta Group at Shinden, Tsuyama City, Okayama Prefecture, south west Japan. Part 1. Description of Perna oyami sp. nov. and its paleoecology. One interesting Perna mytilid is described from the Middle Miocene Katsuta group at Shinden, Tsuyama City, Okayama Prefecture, Southwest Japan: P. oyamai is the 1st record of the genus in Japan. P. oyamai, may have clustered embedded in muddy subs...

Vondracek, Jiri., 2002: On the distribution of the heron Ardea cinerea L in northern Bohemia K rozsireni volavky popelave Ardea cinerea L v severnich Cechach. Fauna Bohemiae Septentrionalis, 27: 77-92

Koyama, T.; Watanabe, H.; Igarashi, G.; Terada, S.; Makabe, S.; Ito, H., 2011: Short-term prognosis of adaptive servo-ventilation therapy in patients with heart failure. This study tested the hypothesis that adaptive servo-ventilation (ASV) therapy improves the prognosis of heart failure (HF) patients, regardless of the severity of sleep-disordered breathing (SDB). 88 consecutive patients were divided into 4 group...

Whitlock, G.; Norton, R.; Clark, T.; Pledger, M.; Jackson, R.; MacMahon, S., 2003: Motor vehicle driver injury and socioeconomic status: a cohort study with prospective and retrospective driver injuries. Study objective: To investigate the association between motor vehicle driver injury and socioeconomic status. Design: Cohort study with prospective and retrospective outcomes. Setting: New Zealand. Participants: 10 525 adults (volunteer sample of...

Brady, H.B., 1881: A On some Arctic Foraminifera from Soundings obtained on the Austro-Hungarian North-Polar Expedition of 1872-74. Annals of Natural History, 5(viil): 393

Otsiogo Oyabi H.; Roblin G., 1985: Uptake of glycine by excised pulvini of mimosa pudica in relation to proton pump activity. Uptake of [3H]-glycine by sections of Mimosa pudica L. pulvini is pH dependent (maximum at pH 5.5) and exhibits biphasic saturation kinetics in the range of concentrations tested (1-75 mM). Effects of compounds which increase [fusicoccin (FC)] or...

Filippov, AG.; Tiunov, MP., 1999: Bat remains in caves of Irkutsk Region. Plecotus et al, 2: 100-107

Andersen K.; Perregaard J.; Arnt J.; Hyttel J.; Sanchez C., 1992: Selective centrally acting serotonin 5 ht 2 antagonists within series of substituted phenylindoles. Abstracts of Papers American Chemical Society 204(1-2): MEDI 86

Briley, M.; Moret, C., 2003: Fibromyalgia syndrome: an overview of potential drug targets. Fibromyalgia syndrome (FMS) is a chronic disease of widespread and debilitating pain. The cause of FMS is unknown and its risk factors are poorly understood. It occurs frequently in the general population where it is often co-morbid with other rhe...

Oprea-Secăreanu, O., 1982: The importance of knowing health conditions in establishing work capacity upon starting work, and at continuing activity. Viata Medicala; Revista de Informare Profesionala Si Stiintifica A Cadrelor Medii Sanitare 30(3): 65-67