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The interaction between CtIP and BRCA1 is not essential for resection-mediated DNA repair or tumor suppression


, : The interaction between CtIP and BRCA1 is not essential for resection-mediated DNA repair or tumor suppression. Journal of Cell Biology 201(5): 693-707

The CtIP protein facilitates homology-directed repair (HDR) of double-strand DNA breaks (DSBs) by initiating DNA resection, a process in which DSB ends are converted into 3'-ssDNA overhangs. The BRCA1 tumor suppressor, which interacts with CtIP in a phospho-dependent manner, has also been implicated in DSB repair through the HDR pathway. It was recently reported that the BRCA1–CtIP interaction is essential for HDR in chicken DT40 cells. To examine the role of this interaction in mammalian cells, we generated cells and mice that express Ctip polypeptides (Ctip-S326A) that fail to bind BRCASurprisingly, isogenic lines of Ctip-S326A mutant and wild-type cells displayed comparable levels of HDR function and chromosomal stability. Although Ctip-S326A mutant cells were modestly sensitive to topoisomerase inhibitors, mice expressing Ctip-S326A polypeptides developed normally and did not exhibit a predisposition to cancer. Thus, in mammals, the phospho-dependent BRCA1–CtIP interaction is not essential for HDR-mediated DSB repair or for tumor suppression.

US$19.90

PMID: 23712259

DOI: 10.1083/jcb.201302145


Other references

Knights, H.T., 1944: Comments on filariasis. From personal experience of infection widi Loa loa acquired in-Africa, Knights draws attention to die fact tiiat a filarial infestation may give rise to a positive Casoni reaction aldiough die complement fixation test for hydatid is negative. A se...

Castro-González, Mía.Isabel.; Pérez-Gil Romo, F., 2012: Chemical composition of eggs of the Olive Ridley Lepidochelys olivacea (Testudines: Cheloniidae) and it's potential as a food source. The Olive Ridley is a worldwide distributed species with high nesting production per season, and in La Escobilla Oaxaca, México, there is a 70% of non-hatched eggs that are lost. In order to evaluate their potential use as a source for human and...

Arvidsson, A.K.; Wraith, A.; Jönsson-Rylander, A.C.; Larhammar, D., 1998: Cloning of a neuropeptide Y/peptide YY receptor from the Atlantic cod: the Yb receptor. Neuropeptide Y (NPY) belongs to a family of structurally related neuroendocrine peptides that bind to G protein-coupled receptors. Five different receptor subtypes have recently been cloned in mammals and we have found another three receptor genes...

Corah, N.L.; Gale, E.N.; Illig, S.J., 1978: Assessment of a dental anxiety scale. A number of different sets of data concerning the Corah Dental Anxiety Scale were evaluated. The data indicate that the scale is a reliable, valid, and useful measure of dental anxiety. It can be successfully used in the dental office or in resear...

Zih, A., 1928: Die hamopoetische Wirkung von ver-futterter Milz. Rabbits bought in the general market were fed a basic diet of hay, oats, and turnips. The rabbits were kept for 1 wk. under as uniform conditions as possible. Red blood cell counts were made by Burker's method. Normal rabbits showed variation...

Luo, J.; Huang, X.; Li, Y.; Li, Y.; Xu, X.; Gao, Y.; Shi, R.; Yao, W.; Liu, J.; Ke, C., 2016: GPR30 disrupts the balance of GABAergic and glutamatergic transmission in the spinal cord driving to the development of bone cancer pain. NlmCategory="UNASSIGNED">Cancer induced bone pain is a very complicated clinical pain states that has proven difficult to be treated effectively due to poorly understand of underlying mechanism, but bone cancer pain (BCP) seems to be...

E.M.daoui, A.; Wu, L.; Wang, R.; de Champlain, J., 2006: Modulation of cardiac and aortic peroxisome proliferator-activated receptor-gamma expression by oxidative stress in chronically glucose-fed rats. Background: The aim of the present study was to examine the chronic effects of a-lipoic acid on proliferator peroxisome activated receptors-gamma (PPAR-gamma) and PPAR-alpha expressions in cardiovascular tissues of chronically hypertensive insulin...

Querido, A., 1987: The discipline called Medicine and the new priorities presented in the 1985 Stimulation of Health Research Program. Nederlands Tijdschrift Voor Geneeskunde 131(7): 278-284

Abate, O.; Bollo, E.; Lotti, D.; Bo, S., 1998: Cytological, immunocytochemical and biochemical cerebrospinal fluid investigations in selected central nervous system disorders of dogs. Cerebrospinal fluid (CSF) obtained from 20 clinically healthy dogs and from 15 dogs affected by neurological disorders were examined for total and differential cell counts, immunocytochemistry for canine distemper virus antigen, total protein conc...

Havea, P., 2006: Protein interactions in milk protein concentrate powders. Solutions (5%, w/w, pH 6.9), made from six different pilot-plant milk protein concentrate (MPC) powders, were centrifuged (700 g for 10 min at 20 degrees C) to isolate the insoluble material. The sediment (insoluble material) was characterized usi...