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Protein encapsulation in biodegradable polymeric nanoparticles: morphology, fluorescence behaviour and stem cell uptake

, : Protein encapsulation in biodegradable polymeric nanoparticles: morphology, fluorescence behaviour and stem cell uptake. Macromolecular Bioscience 13(9): 1204-1212

The synthesis and characterization of new biodegradable polymeric NPs loaded with bovine serum albumin marked with fluorescein isothiocyanate (FITC-BSA) is reported. The protein is encapsulated in poly(D,L-lactide-co-glycolide) (PLGA) NPs by the double emulsion method with subsequent solvent evaporation. The NPs display a spherical shape with a narrow size distribution and no aggregation is observed after drying. Steady-state and time-resolved fluorescence measurements appear to be a sensitive method to investigate the protein environment on the nanometer-scale. Finally, FITC-BSA-loaded NPs are rapidly internalized in stem cells. Interestingly, 25% cells were slightly positive after 28 days.Biodegradable polymeric nanoparticles (NPs) loaded with bovine serum albumin marked with fluorescein isothiocynate (FITC-BSA) are developed, showing a spherical shape and a narrow size distribution. Fluorescence measurements allow the protein macromolecular structure to be analyzed. NPs are rapidly taken up in the stem cells, and, due to the green fluorescence of the FITC, they can be readily monitored.


PMID: 23776101

DOI: 10.1002/mabi.201300140

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