geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Cdc42 coordinates proliferation, polarity, migration, and differentiation of small intestinal epithelial cells in mice


, : Cdc42 coordinates proliferation, polarity, migration, and differentiation of small intestinal epithelial cells in mice. Gastroenterology 145(4): 808-819

Cdc42 is a Rho GTPase that regulates diverse cellular functions, including proliferation, differentiation, migration, and polarity. In the intestinal epithelium, a balance among these events maintains homeostasis. We used genetic techniques to investigate the role of Cdc42 in intestinal homeostasis and its mechanisms. We disrupted Cdc42 specifically in intestinal epithelial cells by creating Cdc42flox/flox-vil-Cre+ and Cdc42flox/flox-Rosa26-CreER+ mice. We collected intestinal and other tissues, and analyzed their cellular, molecular, morphologic, and physiologic features, compared with the respective heterozygous mice. In all mutant mice studied, the intestinal epithelium had gross hyperplasia, crypt enlargement, microvilli inclusion, and abnormal epithelial permeability. Cdc42 deficiency resulted in defective Paneth cell differentiation and localization without affecting the differentiation of other cell lineages. In mutant intestinal crypts, proliferating stem and progenitor cells increased, compared to control mice, resulting in increased crypt depth. Cdc42 deficiency increased migration of stem and progenitor cells along the villi, caused a mild defect in the apical junction orientation, and impaired intestinal epithelium polarity, which may contribute to the observed defective intestinal permeability. The intestinal epithelium of the Cdc42flox/flox-vil-Cre+ and Cdc42flox/flox-Rosa26-CreER+ mice appeared similar to that of patients with microvillus inclusion disease (MVID). In the digestive track, loss of Cdc42 also resulted in crypt hyperplasia in the colon, but not the stomach. Cdc42 regulates proliferation, polarity, migration, and differentiation of intestinal epithelial cells in mice and maintains intestine epithelial barrier and homeostasis. Defects in Cdc42 signaling could be associated with MVID.

US$19.90

PMID: 23792201

DOI: 10.1053/j.gastro.2013.06.021


Other references

Anderson, R.; Kirsop, B., 1974: The control of volatile ester synthesis during the fermentation of wort of high specific gravity. Some effects have been studied of fermenting wort of unusually high specific gravity, followed by dilution with water to give beer of normal original gravity. This procedure permits increased overall rates of beer production, but matching of flavo...

Hofhansl, W.; Baumgarten, K., 1960: The treatment of threatened abortion with gestagens. Wiener Klinische Wochenschrift 72: 853-856

Goldstein, N.S.; Turner, J.R., 2000: Pericolonic tumor deposits in patients with T3, N+, M0 colon adenocarcinomas A marker of reduced disease-free survival and intra-abdominal metastases. Laboratory Investigation 80(3): 81A, March

Tyson; Conradson; Farrow; Jones, 1996: Observation of internal interfaces in PtxCo1-x (x. Physical Review. B, Condensed Matter 54(6): R3702-R3705

Mayo Melendez, Enrique, 1965: Some ecological characteristics of inundated forest in Darien, Panama, and their possible utilization. The field work was done during the dry season (Jan. and Feb. 1965). Four edaphic and one climatic association were studied: a) Avicennia germinans; b) Rhizophora brevistyla; c) Mora oleifera, and d) Prioria copaifera; the climatic association was...

Anonymous, 2016: Editorial. Journal of Pediatric Rehabilitation Medicine 9(4): 251-252

Schmid, T.; Müller, P.; Spelsberg, F., 1997: Parathyroidectomy after renal transplantation: a retrospective analysis of long-term outcome. Background. Advanced hyperparathyroidism refractory to active vitamin D continues to be a problem and frequently forces the nephrologist to resort to parathyroidectomy. One particular aspect is persisting advanced hyperparathyroidism after renal t...

Dalod, M.; Sinet, M.; Deschemin, J.C.; Fiorentino, S.; Venet, A.; Guillet, J.G., 1999: Altered ex vivo balance between CD28+ and CD28- cells within HIV-specific CD8+ T cells of HIV-seropositive patients. The CD8+ CD28- cell population in the blood of HIV-infected individuals is considerably expaneded. Yet the cause of this expansion is not clear. The recent demonstration of identical TCR-rearranged genes in CD8+ Cd28+ and CD8+ CD28- expanded T cel...

Tonelli, M.R.; Aitken, M.L., 2007: Pregnancy in cystic fibrosis. This review summarizes recent knowledge regarding pregnancy in women with cystic fibrosis, including contraception, pre and postpartum medical care and outcomes, as well as reproductive decision-making. Cystic fibrosis women have a fertility rate...

Demuth, D.R.; Irvine, D.C., 2002: Structural and functional variation within the alanine-rich repetitive domain of streptococcal antigen I/II. Members of the antigen I/II family of cell surface proteins are highly conserved, multifunctional adhesins that mediate interactions of oral streptococci with other oral bacteria, with cell matrix proteins (e.g., type I collagen), and with salivar...