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Magnetic separation of encapsulated islet cells labeled with superparamagnetic iron oxide nano particles

, : Magnetic separation of encapsulated islet cells labeled with superparamagnetic iron oxide nano particles. Xenotransplantation 20(4): 219-226

Islet cell transplantation is a promising option for the restoration of normal glucose homeostasis in patients with type 1 diabetes. Because graft volume is a crucial issue in islet transplantations for patients with diabetes, we evaluated a new method for increasing functional tissue yield in xenogeneic grafts of encapsulated islets. Islets were labeled with three different superparamagnetic iron oxide nano particles (SPIONs; dextran-coated SPION, siloxane-coated SPION, and heparin-coated SPION). Magnetic separation was performed to separate encapsulated islets from the empty capsules, and cell viability and function were tested. Islets labeled with 1000 μg Fe/ml dextran-coated SPIONs experienced a 69.9% reduction in graft volume, with a 33.2% loss of islet-containing capsules. Islets labeled with 100 μg Fe/ml heparin-coated SPIONs showed a 46.4% reduction in graft volume, with a 4.5% loss of capsules containing islets. No purification could be achieved using siloxane-coated SPIONs due to its toxicity to the primary islets. SPION labeling of islets is useful for transplant purification during islet separation as well as in vivo imaging after transplantation. Furthermore, purification of encapsulated islets can also reduce the volume of the encapsulated islets without impairing their function by removing empty capsules.


PMID: 23789985

DOI: 10.1111/xen.12042

Other references

Abdazimov U.; Babayev A.G.; Talipov S., 1984: Natural gas deposits; methods of exploration and preparation for exploitation. Uzbekiston Geologiya Zhurnali = Uzbekskiy Geologicheskiy Zhurnal 1984(4): 30-33

McKinney, M., 2011: Attention, please. Reports spotlight quality, patient-safety concerns at nursing homes. Modern Healthcare 41(20): 18-18

Sztriha, L.S.; Nork, M.P.; Abdulrazzaq, Y.M.; al-Gazali, L.I.; Bakalinova, D.B., 1997: Abnormal myelination in peroxisomal isolated dihydroxyacetonephosphate acyltransferase deficiency. The cranial magnetic resonance imaging findings in three siblings with nonrhizomelic chondrodysplasia punctata due to isolated dihydroxyacetonephosphate acyltransferase (DHAP-AT) deficiency are reported. Areas of high signal intensity in a patchy...

Stark, J.C.; Jarrell, W.M., 1980: Salinity-induced modifications in the response of maize to water deficits. Maize was grown in sand culture tanks with -0.4, -2.4, -4.4, or -6.4 bar osmotic potential in a nutrient sol. produced with an equivalent mixture of CaCl2 and NaCl. When the plants were 35 days old, irrigation was stopped and a period of drought s...

Coutinho, J.O.; Rachou, R., 1966: Data on the biology and the malaria vector capacity of anophelines of sub-group Kerteszia under natural conditions. Revista Brasileira de Malariologia E Doencas Tropicais. Publicacoes Avulsas 18(3): 557-580

Gove, R.M., 1953: The role of educational services in an institution for mental defectives. American Journal of Mental Deficiency 57(3): 397-400

Jackson D.W.; Vessels K.J.; Potter D.A., 1981: Resistance of selected cool and warm season turf grasses to the greenbug schizaphis graminum. Three genetically diverse Kentucky bluegrasses (Poa pratensis L. cvs. Kenblue, Vantage and Adelphi) and 6 other turfgrasses were evaluated for susceptibility to the greenbug, S. graminum Rondani. Nine common lawn weed species were tested as potent...

Sterry, W.; Mielke, V.; Konter, U.; Kellner, I.; Boehncke, W.H., 1992: Role of beta 1-integrins in epidermotropism of malignant T cells. To better understand the molecular mechanisms of epidermotropism, we immunohistochemically analyzed the expression pattern of adhesion molecules belonging to the integrin and immunoglobulin superfamilies in cases of mycosis fungoides (MF) (n = 15)...

Yanchuk, S., 2005: Discretization of frequencies in delay coupled oscillators. We study the dynamics of two mutually coupled chaotic oscillators with a time delayed coupling. Due to the delay, the allowed frequencies of the oscillators are shown to be discretized. The phenomenon is observed in the case when the delay is much...

O'Connor, R.; O'Kennedy, R.; James, P., 1995: Use of proton NMR spectroscopy in the toxicological evaluation of new anti-mycobacterial agents. Biochemical Society Transactions 23(2): 357s-357s