+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Epitope screening of the PCV2 Cap protein by use of a random peptide-displayed library and polyclonal antibody

, : Epitope screening of the PCV2 Cap protein by use of a random peptide-displayed library and polyclonal antibody. Virus Research 177(1): 103-107

Capsid protein (Cap), the only structural protein of porcine circovirus 2 (PCV2), is involved in the host protective response and is a target for vaccine development. To find a rapid and easy way to fully map the antigenic epitopes of Cap, purified Cap-specific polyclonal antibodies were used to screen a random heptapeptide phage display library. After three rounds of screening, twenty phage clones that had binding activity to Cap-specific antibodies (tested by phage ELISA) were sequenced. When the inserted amino acid sequences were aligned with the Cap protein sequence, eight core regions in Cap (50SRTFGYT56, 62VRTPSW67, 68AVDMMR73, 79FLPPGG84, 86SNPRSVPF93, 102KVEFWP107, 119GSSXXXLDDN128 and 229PPLNP233) were identified, three of which (50SRTFGYT56, 86SNPRSVPF93 and 102KVEFWP107) for the first time. Nine phagetopes representing the eight regions were chosen to immunize Kunming mice. All except minotopes 50SRTFGYT56 and 229PPLNP233 induced antibodies against PCV2 when injected into Kunming mice.


PMID: 23845304

DOI: 10.1016/j.virusres.2013.06.018

Other references

Dewald, B.W.A.; Sutton, J., 2000: Hospitality employees' empowerment in Hong Kong and the Chinese Mainland. This paper reports on a research project investigating the current and potential existence, implementation and levels of training in empowerment management styles in five international hotel groups in Asia. Initial findings suggest that empowermen...

Wahren, J.; Johansson, B.L.; Ekberg, K., 2000: C-peptide, new discoveries and therapeutic implications. Journees Annuelles de Diabetologie de L'hotel-Dieu: 231-241

Il'inov, M.G.; SokolovskiÄ­, A.M., 1971: Surgical treatment of chronic Monteggia's injuries. Ortopediia Travmatologiia i Protezirovanie 32(4): 53-55

Traulsen, F.; Andersson, J.T.; Ehrhardt, M.G., 1999: Acidic and non-acidic products from the photo-oxidation of the crude oil component dibenzothiophene dissolved in seawater. A solution of dibenzothiophene in membrane-filtered natural seawater was exposed to sunlight in a quartz vessel. The same solution in an amber glass vessel served as the dark control. Acidic photoproducts were isolated, identified and quantified f...

Keltjens, Willem G., 1995: Magnesium uptake by Al-stressed maize plants with special emphasis on cation interactions at root exchange sites. As aluminium (Al) severely inhibits magnesium (Mg) uptake by many plant species, Mg uptake and Mg-Al interactions in maize (Zea mays L.) were studied in a series of short and long-term experiments. A relationship between Mg uptake and the degree o...

Li, M.; Tromp, J.; Zhang, L., 1996: On the nearest neighbour interchange distance between evolutionary trees. We present some new results on a well-known distance measure between evolutionary trees. The trees we consider are free 3-trees having n leaves labeled 0,...,n - 1 (representing species), and n - 2 internal nodes of degree 3. The distance between...

Shapiro A.; Boyarsky S., 1984: Effects of drugs on the ureter and renal pelvis. Caine, M (Ed ) Clinical Practice in Urology: The Pharmacology Of The Urinary Tract Xii+167p Springer-Verlag: Berlin, West Germany; New York, N Y , Usa Illus 69-76

Teague, D.J.; Sheehan, D.J., 2009: The Pachuco mark and similar tattoos. Southern Medical Journal 102(1): 108-109

Tsai, T-Jung.; Chouillard, E.K.; Gumbs, A.A., 2012: Laparoscopic right hepatectomy with intrahepatic transection of the right bile duct. Although our earlier videos demonstrated extrahepatic control of the hepatic arterial, portal venous, and biliary system, we have begun transecting the biliary system intraparenchymally for lesions distant from hilar plate and the confluence of th...

Pisarczyk J.M.; Splittstoesser W.E., 1979: Response of tomato to pre transplanting applications of chlormequat daminozide and ethephon. Succinic acid-2,2-dimethylhydrazide (daminozide), (2-chloroethyl)trimethylammonium chloride (chlormequat), or (2-chloroethyl)phosphonic acid (ethephon) inhibited the growth of tomato (Lycopersicon esculentum Mill.) plants for 2 wk when applied at...