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Ultrastructure and Populational Ratios of Hemocytes of the Brown Dog Tick, Rhipicephalus sanguineus Latreille Ixodoidea, Ixodidae


, : Ultrastructure and Populational Ratios of Hemocytes of the Brown Dog Tick, Rhipicephalus sanguineus Latreille Ixodoidea, Ixodidae.

Identification of Hemocytes is essential to understand hemocyte-mediated immune responses in invertebrates. The ultrastructure and characteristics of hemocytes of field-collected semiengorged female dog ticks, Rhipicephalus sanguineus (Latreille) are herein presented on the basis of hemocyte types, morphological cell features and ultrastructural characteristics of cytoplasmic inclusions. Samples of haemolymph were obtained by cutting the caudal alloscutum and collecting the drop there formed. The samples were fixed and stained with Giemsa solution for light microscopic examination. Ultra-thin sections were prepared, stained and examined by TEM. Light and TE Microscopy observation resulted in the characterization of three basic cellular types: Plasmatocytes (pL), granular hemocytes (Gr) and prohemocytes (ph). Comparison of cell type population ratio showed pL as the most abundant hemocyte (57%), followed by Gr (30%). ph accounted for only 12% of the hemocyte population.

US$19.90

DOI: 10.3923/jp.2013.64.70


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