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Association Mapping of Insecticide Resistance in Wild Anopheles gambiae Populations Major Variants Identified in a Low-Linkage Disequilbrium Genome


, : Association Mapping of Insecticide Resistance in Wild Anopheles gambiae Populations Major Variants Identified in a Low-Linkage Disequilbrium Genome. PLoS ONE 5(10): e13140

Association studies are a promising way to uncover the genetic basis of complex traits in wild populations. Data on population stratification, linkage disequilibrium and distribution of variant effect-sizes for different trait-types are required to predict study success but are lacking for most taxa. We quantified and investigated the impacts of these key variables in a large-scale association study of a strongly selected trait of medical importance: pyrethroid resistance in the African malaria vector Anopheles gambiae. Methodology/Principal Findings: We genotyped appr. equal 1500 resistance-phenotyped wild mosquitoes from Ghana and Cameroon using a 1536-SNP array enriched for candidate insecticide resistance gene SNPs. Three factors greatly impacted study power. (1) Population stratification, which was attributable to co-occurrence of molecular forms (M and S), and cryptic within-form stratification necessitating both a partitioned analysis and genomic control. (2) All SNPs of substantial effect (odds ratio, OR>2) were rare (minor allele frequency, MAF<0.05). (3) Linkage disequilibrium (LD) was very low throughout most of the genome. Nevertheless, locally high LD, consistent with a recent selective sweep, and uniformly high ORs in each subsample facilitated significant direct and indirect detection of the known insecticide target site mutation kdr L1014F (OR appr. equal 6; P<10-6), but with resistance level modified by local haplotypic background. Conclusion: Primarily as a result of very low LD in wild A. Gambiae, LD-based association mapping is challenging, but is feasible at least for major effect variants, especially where LD is enhanced by selective sweeps. Such variants will be of greatest importance for predictive diagnostic screening.

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Other references

Conte J.V.; Foegh M.L.; Calcagno D.; Cai B.R.; Ramwell P.W.; Wallace R.B., 1989: Inhibition of angioplasty induced myointimal hyperplasia with angiopeptin. Surgical Forum (Chicago) 40: 302-303

Patil, V.S.; Magar, N.G., 1959: Relation of dietary fat and cholesterol to tissue and serum cholesterol and polyunsaturated fatty acids in rats. Cholesterol-phospholipids and fatty acids were estimated in liver, kidney, heart, brain and serum of rats fed coconut oil, hydro-genated fat, butter fat and groundnut oil at 5% level with and without cholesterol for 10 months. Polyunsaturated fatt...

Ovadia, B.; Bekker, J.M.; Fitzgerald, R.K.; Kon, A.; Thelitz, S.; Johengen, M.J.; Hendricks-Munoz, K.; Gerrets, R.; Black, S.M.; Fineman, J.R., 2002: Nitric oxide-endothelin-1 interactions after acute ductal constriction in fetal lambs. Acute partial compression of the fetal ductus arteriosus (DA) results in an initial increase in pulmonary blood flow (PBF) that is followed by acute vasoconstriction. The objective of the present study was to determine the role of nitric oxide (NO...

Kimura, Y.; Hyogo, H.; Yamagishi, S-ichi.; Takeuchi, M.; Ishitobi, T.; Nabeshima, Y.; Arihiro, K.; Chayama, K., 2010: Atorvastatin decreases serum levels of advanced glycation endproducts (AGEs) in nonalcoholic steatohepatitis (NASH) patients with dyslipidemia: clinical usefulness of AGEs as a biomarker for the attenuation of NASH. Advanced glycation endproducts (AGEs), final reaction products of protein with sugars, are known to contribute to various disorders, including diabetes, aspects of aging, and neurodegenerative diseases. Recently, we reported elevated levels of ser...

Kiejna, A.; Rymaszewska, J.; Hadryś, T.; Suwalska, A.; Łojko, D.; Rybakowski, J.K., 2006: Bipolar or unipolar? - the question for clinicians and researchers. Background: Correct diagnosis and criteria of affective disorders is always a subject of interest to researchers and practitioners.Methods: The study aimed at assessing frequency of various traits and symptoms of bipolar affective disorders (BP-I,...

Akimasa Masuda, 1968: Geochemistry of lanthanides in basalts of central Japan. Lanthanides in six basalts from Central Japan have been determined by a stable isotope dilution method. The chondrite-normalized lanthanide patterns are discussed in terms of partition coefficients estimated by Masuda and Matsui [1]. An areal rela...

Ellis R., 1976: Kinetics of chlorophyll synthesis in the green alga golenkinia. Plant Physiology (Rockville) 57(5 SUPPL): 72

Accolla, E.A.; Dukart, J.; Helms, G.; Weiskopf, N.; Kherif, F.; Lutti, A.; Chowdhury, R.; Hetzer, S.; Haynes, J-Dylan.; Kühn, A.A.; Draganski, B., 2015: Brain tissue properties differentiate between motor and limbic basal ganglia circuits. Despite advances in understanding basic organizational principles of the human basal ganglia, accurate in vivo assessment of their anatomical properties is essential to improve early diagnosis in disorders with corticosubcortical pathology and opt...

Budzyńska-Włodarczyk, J.; Michalska-Jakubus, Młgorzata.M.; Kowal, Młgorzata.; Krasowska, D., 2016: Evaluation of serum concentrations of the selected cytokines in patients with localized scleroderma. Localized scleroderma is an autoimmune disease primarily affecting the skin. The cause of disease remains unexplained although environmental factors are implicated, which are likely to be responsible for activation of the endothelium and subsequen...

Clyne, D., 1973: Notes on the web of Poecilopachys australasia (Griffith and Pidgeon, 1833) (Araneida: Argiopidae). Australian entomological magazine: 1 (3) 23-39