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31P-nuclear magnetic resonance studies of intact plasmodia of Physarum polycephalum

, : 31P-nuclear magnetic resonance studies of intact plasmodia of Physarum polycephalum. FEBS (Federation of European Biochemical Societies) Letters, 1761: 161-165

31P-nuclear magnetic resonance spectra were obtained from intact plasmodial cells of Physarum polycephalum, where cytoplasmic streaming is generated by actin-myosin-ATP interaction. Several peaks were resolved and identified. They included ATP, ADP, orthophosphate and polyphosphates. Peaks for phosphocreatine, phosphoarginine or AMP were not detected. The intracellular pH and concentrations of ATP and free Mg2+ were estimated to be pH 6.9, 0.2–0.5 mM, and about 1 mM, respectively.


DOI: 10.1016/0014-5793(84)80932-1

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