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Evidence that bovine conglutinin reacts with an early product of C3b degradation, and an improved conglutination assay

, : Evidence that bovine conglutinin reacts with an early product of C3b degradation, and an improved conglutination assay. Journal of Immunology 121(2): 658-664

When EAC43b were treated with heated serum in EDTA, reactivity with bovine conglutinin appeared rapidly, even at 0 degrees C, and almost simultaneously with the loss of C3b rosetting capacity. At the time conglutinability first appeared, there was no detectable decrease in I-A or hemolytic C3 activity, and no detectable C3 antigen release from the cells. With prolonged exposure to heated serum in EDTA, I-A (immune adherence) and hemolytic C3 activity were lost. If this exposure was at 37 degrees C, C3 antigen became strongly detectable in the supernatant fluid, and eventually conglutinability was markedly reduced or lost, whereas C3d rosettes were unaffected. We suggest that bovine conglutinin reacts with some early product of C3b degradation, rather than with C3d, and propose that this intermediate be designated C3k. We have developed a semi-quantitative assay for bovine conglutinin, utilizing a Coulter Counter to register the decrease in total particles due to red cell aggregation. By using this method, we have detected conglutination with mouse complement (C) as well as with that from man and the guinea pig.


PMID: 98586

Other references

D.M.B.stos, F.; Dos Santos, A.G.; Jones, J., J.; Oestreicher, E.G.; Pinto, G.F.; Paiva, L.M.C., 1999: Three different coupled enzymatic systems for in situ regeneration of NADPH. Three different coupled enzymatic systems used in the reduction of sulcatone by alcohol dehydrogenase from Thermoanaerobium brockii (TBADH), were kinetically compared. The first one involved the use of TBADH for both the principal and recycling re...

Maroteaux, P., 1970: Biochemical differentiation of Hurler's and Hunter's diseases by fracionation of heparitin sulfate. Revue Europeenne D'etudes Cliniques et Biologiques. European Journal of Clinical and Biological Research 15(2): 203-205

Karczewski A., 1974: Structural features of kame forms as an expression of the dynamics of morphogenetic environment. Quaestiones Geographicae 1): 53-63

Carbonell, F.; Grilli, G., 1982: Erythroid and myeloid stem cells: values obtained from normal subjects with the methyl-cellulose technique. Sangre 27(1): 15-18

Henriques, T.; Adelman, K., 2013: Catching the waves: following the leading edge of elongating RNA polymerase II. By precisely tracking the waves of elongating RNA polymerase II (Pol II) during gene activation, Danko et al. (2013), in this issue of Molecular Cell, discovered a surprising diversity of elongation rates among and along human genes.

Eisenberg, H., 1978: A new malpractice carrier--for good doctors. Medical Economics 55(2): 136-9, 142-3, 146

Wallace, A.B., 1975: Alexander Burns Wallace. Lancet 1(7897): 52-53

Mcdaniel C.A.; Howard R.W.; Blomquist G.J.; Collins A.M., 1983: Hydro carbons of the cuticle sting apparatus and sting shaft of apis mellifera identification and preliminary evaluation as chemo taxonomic characters. The hydrocarbon components of the cuticle, sting apparatus and sting shaft of foragers of A. mellifera L. were identified and quantitated. Major qualitative and quantitative differences were found between the cuticular hydrocarbon components and t...

Janz, G.J.; Pinto, G.L.; Franca, C.S.; Barbosa, J.C.L., 1955: State of nutrition and infestation with ankylostomes. The faeces of 100 apparently healthy African subjects were examined for ankylostome eggs. Three groups were selected, consisting of 42 subjects probably without parasites and 55 with parasites, of whom 13 seemed to be most heavily infested. Haemat...

Rosen, H.R.; Larsh, H.W., 1946: Blight of oats caused by an Helminthosporium differing from the one producing leaf spot. An abstract.