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Feulgen, iron-propionocarmine and cupra-ammonium in preparing algal chromosomes for light microscopy


, : Feulgen, iron-propionocarmine and cupra-ammonium in preparing algal chromosomes for light microscopy. Stain Technology 54(3): 129-133

A method for obtaining algal chromosomal preparations is described employing the Feulgen method for DNA staining, Fe-propionocarmine as an enhancing stain, and cupra-ammonium to remove cell wall material. Fe-propionocarmine applied as a gradient to the side provides cells stained with the Feulgen stain alone or with the Feulgen Fe-propionocarmine stain, thereby facilitating useful comparison. Where dilute the Fe-propionocarmine enhances nuclear staining without staining orthe organelles; where more concentration it also stains the nucleolus, spindle, spindle polar bodies, pyrenoid and protoplast. Treatment with cupra-ammonium, to remove polysaccharide wall material, followed by neutralization with propionocarmine, enables thinner squashes and better chromosome spreads without loss of differential staining. Preparations mounted in euparal are long-lasting.

US$29.90

PMID: 91242


Other references

Celuch, S.M.; Sloley, B.D., 1988: Regional distribution of dopamine, 5-hydroxytryptamine, and noradrenaline in the rat vas deferens. The concentrations of dopamine (DA), 5-hydroxytryptamine (5-HT) and noradrenaline (NA) in the rat vas deferens divided in eight or four sections were determined by high performance liquid chromatography with electrochemical detection. Dopamine and...

Polakoff, S., 1988: Decrease in acute hepatitis B incidence continued in 1987. Lancet 1(8584): 540-540

Cable, M.B.; Jacobus, J.; Powell, G.L., 1978: Cardio lipin a stereospecifically spin labeled analog and its specific enzymic hydrolysis. The spin-labeled bovine cardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-(16-doxylstearoyl)glycero(3)phospho]-sn-glycerol was prepared. The stereoselective synthesis makes use of the monolysocardiolipin 1-(3-sn-phosphatidyl)-3-[1-acyl-2-lyso-sn-glyce...

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Lindhe J.; Nyman S., 1980: Alterations of the position of the marginal soft tissue following periodontal surgery. Alterations of the position of the marginal soft tissue (gingival margin) on the buccal surface of teeth in patients who, following peridontal surgery, were enrolled in a supervised maintenance care program for 10-11 yr were examined. The material...

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Rosenfield, R.E.; Diamond, S.H., 1981: Diagnosis and treatment of the immune hemolytic anemias. Immune hemolytic anemia can be treated by blood transfusion, steroids, cytotoxic drugs, plasmapheresis, and splenectomy. However, the benefits of therapy are dependent upon the relationship of treatment to the etiology of disease. Thus, effective...

Chen, B.; Fu, J-feng.; Yuan, W-hong.; Xu, Y.; Luo, Z-hang.; Wang, H.; Chen, Z-hua.; Li, C-ping., 2005: Study on the mechanism of protective effect of oral L-arginine on intestine after scald injury in rats. To explore the mechanism of protective effect of oral L-arginine (L-Arg) on the intestine after scald injury in rats. Sixty-six Sprague-Dawley (SD) rats were randomly divided into three groups: i.e. normal control (N, n = 6, without treatment), or...

Weiss William W.; Broadhead Ronald F.; Balch Robert S.; W.S.aochang; Ruan Tongjun, 2001: Artificial intelligence to reduce the risk of oil exploration and production. The patterns that are evident in geophysical logs or 3D seismic surfaces are fuzzy sets. A ranking technique based on fuzzy logic is applied to prioritize the relationship between fuzzy sets and oil production. Neural networks are used to correlat...

Saini, A.S., 1967: Some technical improvements in the paper chromatography of keto acid 2,4-dinitrophenylhydrazones. Journal of Chromatography 26(2): 539-541