geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Hydroxymethylglutaryl-coenzyme A reductase. Purification and properties of the enzyme from Fusarium oxysporum


, : Hydroxymethylglutaryl-coenzyme A reductase. Purification and properties of the enzyme from Fusarium oxysporum. Biochimica et Biophysica Acta 523(2): 283-296

The hydroxymethylglutaryl-coenzyme A reductase (mevalonate:NADP+ oxidoreductase, EC 1.1.1.34) system in Fusarium oxysporum, a soil inhabiting plant pathogen, has been examined. Two forms of the enzyme catalyzing the conversion of hydroxymethylglutaryl-coenzyme A were obtained in the supernatant after precipitation at 75% (NH4)2SO4 saturation of the soluble culture extract which was previously separated from cell wall, mitochondria and microsomes. The two forms of the enzyme were separated electrophoretically. A third form, contained in the precipitate obtained at 35--75% (NH4)2SO4 saturation of the same extract, was further purified by Sephadex G-50 column chromatography. This purified form moved as a single band in sodium dodecyl sulphate electrophoresis and in immunological tests and has a molecular weight of 11 000. The apparent Michaelis constant for the substrate hydroxymethylglutaryl-coenzyme A is 21 micron at 2 micron NADP. NADPH is a more efficient reductant on a molar basis than NADH for the deacylation of the hydroxymethylglutaryl-coenzyme A substrate. Optimum activity of the enzyme was obtained at pH 7.4 and 37 degrees C. The enzyme demonstrated no cold sensitivity but rather was more stable at 4 degrees C than at 25 degrees C. The protection with dithiothreitol, though minimal compared to other systems, was more effective at the higher temperature.

US$29.90

PMID: 26407


Other references

Ruthmann, A., 1976: Cytologische und cytophotometrische Untersuchungen am Makronucleus eines hypotrichen Ciliaten der Gattung Oxytrichla. Archiv Protistenk18(4): 268-284

Stuart, J.J.; Hatchett, J.H., 2009: Cytogenetics of the Hessian fly: I. mitotic karyotype analysis and polytene chromosome correlations. The somatic haploid chromosome set of the Hessian fly, Mayetiola destructor (Say),consists of two autosomes and two sex chromosomes. Female somatic cells have pairs of each of these chromosomes (2n = 8). Male somatic cells have two pairs of autoso...

Rönner, A-Clara.; Lindmark, H., 2007: Quantitative detection of Campylobacter jejuni on fresh chicken carcasses by real-time PCR. Campylobacter jejuni infection is a significant cause of foodborne gastroenteritis worldwide. Consumption and handling of poultry products is believed to be the primary risk factor for campylobacteriosis. Risk assessments require quantitative dat...

Heydari, N.D.s-Gupta, A.L.of, R., 2001: Salinity and sodicity influences on infiltration during surge flow irrigation. The interactive influences of water quality and surge-flow irrigation (intermittent application of water) on infiltration into a bare loam soil, packed into a long metal flume, were measured with a laboratory recirculating infiltrometer devised fo...

Gutzke E.R.; Reed J.R.; Grant G.C., 1973: The influence of nitrilo tri acetic acid on heavy metal ion uptake in blue gills lepomis macrochirus. Virginia Journal of Science 24(3): 145

Voiers, D., 2002: The one-visit/one-hour crown. Dentistry Today 21(9): 64-67

Cappelletto, Anna, 1933: A sp of Italian Thysanoptera with note on the probable existence of males in species held as parthenogenetic. Taeniothrips arcangelii [female] [male] (p.326), Italy, on Iris germanica, Ranunculus, Leucan-themum, Salvia, and Nasturtium. The author propounds the hypothesis that in species in which the [male] is not known, this is not necessarily because the...

Fujita, A.; Okamoto, T.; Nose, Y., 1954: Antithiamine factors of ferns. The nature of antivitamin B1factors in Japanese fern was investigated with extracts obtained from dried fern with a phosphate buffer at pH7 and a temperature below 60 degrees C. The thermolabile antivitamin B1factor was inactivated by heating at 1...

Su, H.; Gorodny, N.; Gomez, L.Felipe.; Gangadharmath, U.; Mu, F.; Chen, G.; Walsh, J.C.; Szardenings, K.; Kolb, H.C.; Tamarappoo, B., 2015: Noninvasive molecular imaging of apoptosis in a mouse model of anthracycline-induced cardiotoxicity. Anthracycline-induced cardiotoxicity and myocardial dysfunction may be associated with apoptosis. Caspase 3 catalyzes a terminal step in apoptosis, and its expression may serve as a marker of cardiomyocyte apoptosis. We synthesized 18F-CP18, a cas...

Shadan, F.F.; Riccardiello, L.; Goel, A.; Smith, W.; Chang, D.K.; Boland, R., 2001: Normal human colon epithelial cells propagated in long-term cell cultures support JC polyomavirus infection. Gastroenterology 120(5 Supplement 1): A 664, April