+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Influence of cysteamine on differential staining of BUdR-substituted human chromosomes

, : Influence of cysteamine on differential staining of BUdR-substituted human chromosomes. Canadian Journal of Genetics and Cytology. Journal Canadien de Genetique et de Cytologie 21(1): 145-149

In 5-bromodeoxyuridine (BUdR)-substituted human chromsomes stained with 4'-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.


PMID: 89890

Other references

Redoble, T.G., 1983: The human resources development program of the National Manpower and Youth Council for Muslims of Region X. The findings disclosed that the majority of the participants had attained intermediate and secondary levels of education. Before the training, 43% of the respondents were jobless. After the training, 19% were immediately employed either by the gov...

Allen, J.E.; Macdonald, A.S., 1998: Profound suppression of cellular proliferation mediated by the secretions of nematodes. Loss of T lymphocyte proliferation and the emergence of a host response that is dominated by a Th2-type profile are well-established features of human filarial infection. Down-regulation and modulation of host T cell responses during lymphatic fil...

Yang X.H.; Knapp R.M.; Simpson R.P., 1996: Reservoir simulation of a Prue reservoir, Long Branch Field, Payne County, Oklahoma. Special Publication - Oklahoma Geological Survey Pages 114-118

Anonymous, 1942: Final Report of Nutritional Investigations in Mauritius, 1942-45. Obtainable from the Crown Agents and the Government Printer, Mauritius. 32. This report embodies the result of investigations carried out by the Nutrition Research Unit, formed primarily to advise Government on wartime nutritional problems during the period 1942-45. The report is divided into four sections: (a) Survey...

Samanez-Larkin, G.R., 2013: Financial Decision Making and the Aging Brain. Aps Observer 26(5): 30-33

Chausmer A.B.; Colucci A.V., 1973: Trans membrane transport of trace elements nickel and mercury. Environmental Health Perspectives 4: 101-102

Liu, Y-Chen.; Li, J-Rong.; Sun, C-Hu.; Andrews, E.; Chao, R-Fang.; Lin, F-Mao.; Weng, S-Long.; Hsu, S-Da.; Huang, C-Chen.; Cheng, C.; Liu, C-Chi.; Huang, H-Da., 2016: CircNet: a database of circular RNAs derived from transcriptome sequencing data. Circular RNAs (circRNAs) represent a new type of regulatory noncoding RNA that only recently has been identified and cataloged. Emerging evidence indicates that circRNAs exert a new layer of post-transcriptional regulation of gene expression. In t...

Chan, S.C.uan; Chang, Y.S.iun; Kuo, S.C.u, 1997: Neoflavonoids from Dalbergia odorifera. Two new neoflavonoids, 3'-hydroxymelanettin and 3'-hydroxy-2,4,5-trimethoxydalbergiquinol, were isolated from the heartwood of Dalbergia odorifera, together with the known compounds, dalbergin, melanettin, stevenin, 4-methoxydalbergione,...

Hellenbrecht, D.; Lemmer, B.; Wiethold, G.; Grobecker, H., 1973: Measurement of hydrophobicity, surface activity, local anaesthesia, and myocardial conduction velocity as quantitative parameters of the non-specific membrane affinity of nine -adrenergic blocking agents. Naunyn-Schmiedeberg's Archives of Pharmacology 277(2): 211-226

Guerrier, Y., 1962: Chromophobe adenoma of the pituitary with nasopharyngeal involvement. Les Annales D'oto-Laryngologie 79: 766-769