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Influence of cysteamine on differential staining of BUdR-substituted human chromosomes


, : Influence of cysteamine on differential staining of BUdR-substituted human chromosomes. Canadian Journal of Genetics and Cytology. Journal Canadien de Genetique et de Cytologie 21(1): 145-149

In 5-bromodeoxyuridine (BUdR)-substituted human chromsomes stained with 4'-6-diamidino-2-phenylindole (DAPI) differential staining is suppressed totally by the H+-donor cysteamine (concentration 0.08 M). We propose that differential staining appears because the double BUdR-substituted chromatid will be disintegrated via a photosensitive dye-visible light system. It is suggested that cysteamine prevents the production of strand breaks in DNA and, consequently, differential staining in BUdR-substituted chromosomes. Furthermore it is shown that differential staining with DAPI causes irreversible changes in the double BUdR-substituted chromatid. This finding can be explained with the above mentioned mechanism.

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PMID: 89890


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