geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Pyridoxal phosphatase: cytochemical localization in GERL and other organelles of rat neurons


, : Pyridoxal phosphatase: cytochemical localization in GERL and other organelles of rat neurons. Journal of Histochemistry and Cytochemistry 26(10): 809-821

A phosphatase, hydrolyzing pyridoxal-5-phosphate (P5P), a physiologically active component of the vitamin B6 complex and an essential co-enzyme in the synthesis of neurotransmitters, has been localized cytochemically in the perikarya of neurons in the peripheral, autonomic and central nervous systems of the rat. Neurons in dorsal root ganglia, sympathetic ganglia and ventral horn of spinal cord were studied by light and electron microscopy, while Purkinje cells, neurons in the dentate nucleus of the cerebellum, thalamus, and hypothalamus were studied by light microscopy only. Optimal conditions for demonstrating this activity in aldehyde-fixed tissue were determined with dorsal root ganglia. At the optimal pH of 5.0, neurons in these ganglia and in all other neurons studied show pyridoxal-5-phosphatase (P5Pase) activity in GERL. Small neurons in dorsal root ganglia also display enzyme activity in the endoplasmic reticulum (ER); activities in GERL and ER are also appreciably high at neutral pH. Small and large neurons in these ganglia, and neurons of sympathetic ganglia, show variable P5Pase activity in the Golgi apparatus. These localizations differ from the usual sites of both acid phosphatase and alkaline phosphatase activities. The P5Pase activity, demonstrated cytochemically, is a new acid hydrolase activity in GERL.

US$29.90

PMID: 31396


Other references

Low J.C., 1987: The electroretinogram of heterozygous positive rd mice in vivo. Journal of Physiology (Cambridge) 382: 116P

Hoff-Jorgensen, E., 1966: Antivitamins for B6. Bibliotheca Nutritio et Dieta 8: 160-168

Kirkham, B.W.; Lassere, M.N.; Edmonds, J.P.; Juhasz, K.M.; Bird, P.A.; Lee, C.Soon.; Shnier, R.; Portek, I.J., 2006: Synovial membrane cytokine expression is predictive of joint damage progression in rheumatoid arthritis: a two-year prospective study (the DAMAGE study cohort). Objective. The primary aim of this prospective 2-year study was to explain the wide variability in joint damage progression in patients with rheumatoid arthritis (RA) from measures of pathologic changes in the synovial membrane.Methods. Patients u...

Yu, H.S.; Li, Y.L.ng; Wu, C.S.an, 1998: Purified immunoglobulin G from non-segmental-type vitiligo induces melanocytotoxicity via a diverse spectrum of immune mechanisms. Journal of Dermatological Science 16(SUPPL 1): S101, March

Weinstein, D.; Leininger, J.; Hamby, C.; Safai, B., 2014: Diagnostic and prognostic biomarkers in melanoma. Melanoma is a lethal melanocytic neoplasm. Unfortunately, the histological diagnosis can be difficult at times. Distinguishing ambiguous melanocytic neoplasms that are benign nevi from those that represent true melanoma is important both for treat...

Rivest R.; Jolicoeur F.B.; Marsden C.A., 1990: Discrimination between typical and atypical neuroleptics in vivo with amfonelic acid. European Journal of Pharmacology 183(5): 1887

Wirt, S.E.; Sage, J., 2010: p107 in the public eye: an Rb understudy and more. p107 and its related family members Rb and p130 are critical regulators of cellular proliferation and tumorigenesis. Due to the extent of functional overlap within the Rb family, it has been difficult to assess which functions are exclusive to in...

Burnet, N.G.; Taylor, R.E., 1997: Malignant cerebral glioma. Modern radiotherapy techniques are needed to spare normal brain tissue. Bmj 314(7084): 900-901

Gobert, D.; Schechter, M.; Schollwöck, U.; von Delft, J., 2004: Well-defined quasiparticles in interacting metallic grains. We analyze spectral functions of mesoscopic systems with large dimensionless conductance, which can be described by a universal Hamiltonian. We show that an important class of spectral functions are dominated by one single state only, which implie...

Nagamoto, Y.; Iwasaki, M.; Sugiura, T.; Fujimori, T.; Matsuo, Y.; Kashii, M.; Sakaura, H.; Ishii, T.; Murase, T.; Yoshikawa, H.; Sugamoto, K., 2014: In vivo 3D kinematic changes in the cervical spine after laminoplasty for cervical spondylotic myelopathy. Cervical laminoplasty is an effective procedure for decompressing the spinal cord at multiple levels, but restriction of neck motion is one of the well-known complications of the procedure. Although many authors have reported on cervical range of...