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Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules


, : Sites within gene lacZ of Escherichia coli for formation of active hybrid beta-galactosidase molecules. Journal of Bacteriology 139(1): 13-18

We describe the genetic analysis of 21 Escherichia coli strains in which the amino-terminal sequence of beta-galactosidase has been removed and replaced by an amino-terminal sequence from one or another of the proteins involved in maltose transport. Genetic mapping of the lacZ end of these fused genes indicates that only those fusions in which fewer than 41 amino acids are removed from the amino-terminal sequence of beta-galactosidase result in enzymatically active molecules. Within the region between amino acid 17 and amino acid 41 there are at least four or five sites where enzymatically active hybrid proteins can be formed.

US$29.90

PMID: 110776


Other references

Grant Todd, 1998: Electromagnetic coupling for tensor reconnaissance IP surveying. Preview 76: 114

Sterling J., 1985: Perkin elmer pursues biotech supply market via laboratory robotics. Genetic Engineering News 5(8): 19, 42

Duke-Elder, P.M.; Duke-Elder, W.S., 1931: Studies on the intra-ocular pressure: Part II. The physico-chemical factors controlling the intra-ocular pressure. Journal of Physiology 71(3): 268-274

Claudon, A., 1934: Le Milan royal, Milvus milvus milvus dans le department des Vosges. Alauda Paris, 6: 528-531

Deviataĭkin, E.V.; Abramov, I.V., 1987: Statistical characteristics of radiation control data. Gigiena i Sanitariia: 25-28

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