+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Specific phospholipid requirement for activity of the purified respiratory chain NADH dehydrogenase of Escherichia coli

, : Specific phospholipid requirement for activity of the purified respiratory chain NADH dehydrogenase of Escherichia coli. Biochimica et Biophysica Acta 487(2): 368-377

The highly purified respiratory chain NADH dehydrogenase (EC of Escherichia coli is inactive in the absence of detergent or phospholipid. Triton X-100 is the detergent that gives optimal activity, but the Triton X-100-activated enzyme is stimulated an additional 2-fold by E. coli phospholipids. Phosphatidylglycerol and diphosphatidylglycerol are the most effective lipid activators. The activated complex prepared with diphosphatidylglycerol is stable, whereas that with phosphatidylglycerol loses activity rapidly. Maximum activation by phospholipids occurs after preincubation at 0 degrees C and at pH 7. Triton X-100 is required at low concentrations for lipid activation, but high concentrations interfere with the activation. When the enzyme is optimally activated by phospholipids, it may be additionally activated 2-fold by spermidine, but not by magnesium. In contrast, the Triton X-100-activated form of the enzyme is stimulated by several divalent cations, without specificity. Thus, the most stable, active form of the purified NADH dehydrogenase is generated in the presence of diphosphatidylglycerol and spermidine.


PMID: 16660

Other references

Samuel; Bhandari, 1988: General setting for Berry's phase. Physical Review Letters 60(23): 2339-2342

Belcher, P.; Ross, D., 1991: Aortic root replacement--20 years experience of the use of homografts. The aortic root was replaced with a free root homograft 100 times in 91 patients from 1970 to January 1987. The ages of the patients were in the range 5-73 years (mean 29.7). There were 63 males and 34 females. Thirty had endocarditis with root ab...

Zhong, Q.; Zhou, B.; Ann, D.K.; Minoo, P.; Liu, Y.; Banfalvi, A.; Krishnaveni, M.S.; Dubourd, M.; Demaio, L.; Willis, B.C.; Kim, K-Jin.; duBois, R.M.; Crandall, E.D.; Beers, M.F.; Borok, Z., 2011: Role of endoplasmic reticulum stress in epithelial-mesenchymal transition of alveolar epithelial cells: effects of misfolded surfactant protein. Endoplasmic reticulum (ER) stress has been implicated in alveolar epithelial type II (AT2) cell apoptosis in idiopathic pulmonary fibrosis. We hypothesized that ER stress (either chemically induced or due to accumulation of misfolded proteins) is...

Alkalai, A., 1976: Study of plant stabilizers for m. Selskostopanska tekhnika: 3 (7) 43-51

Musci, R.J.; Hart, S.R.; Ialongo, N., 2015: Internalizing antecedents and consequences of binge-eating behaviors in a community-based, urban sample of African American females. The etiology of problem-eating behaviors is often overlooked in research as it typically shares many symptoms with other more common psychiatric illnesses. Binge-eating problems are at the forefront of the popular media because of the connection t...

Rozsíval, P.; Aschenbrennerová, J., 1994: A favorable outcome after expulsive hemorrhage in cataract surgery via a tunnel incision. The authors refer about expulsive haemorrhage during cataract surgery done by tunnel incision in 73 years old women. She was on long term treatment for glaucoma and arterial hypertension. Three months after the surgery visual acuity was 5/7.5 with...

Sultana, A.; Sridhar, M.S.; Jagannathan, A.; Balasubramanian, D.; Kannabiran, C.; Klintworth, G.K., 2004: Novel mutations of the carbohydrate sulfotransferase-6 (CHST6) gene causing macular corneal dystrophy in India. Macular corneal dystrophy (MCD) is an autosomal recessive disorder characterized by progressive central haze, confluent punctate opacities and abnormal deposits in the cornea. It is caused by mutations in the carbohydrate sulfotransferase-6 (CHST6...

Wang, 1992: Dynamics of plane-symmetric thin walls in general relativity. Physical Review. D, Particles and Fields 45(10): 3534-3543

Kiczek, A.P.; Wong, M., 2001: Method for encapsulating reactive dental agents. A method for encapsulating a water soluble reactive agent in an alkyl cellulosic polymer to provide an encapsulated product from which leaching of the reactive agent into an aqueous dentifrice containing another compound reactive with the agent is...

Loncar D.; Cannon B.; Nedergaard J.; Bedrica L.; Afzelius B.A., 1987: The adipose tissue young cats after cold stress apparent morphological transformation from white to brown adipose tissue. Acta Anatomica 130(1): 54