+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Wall-associated protein antigens of Streptococcus mutans

, : Wall-associated protein antigens of Streptococcus mutans. Journal of General Microbiology 114(1): 109-115

When heat-killed whole organisms of Streptococcus mutans strain Ingbritt (serotype c) were injected into rabbits, antibodies to at least 12 antigens were detectable by crossed immunoelectrophoresis. In contrast, when rabbits were immunized with organisms which had been subjected to extraction with the detergent sodium dodecyl sulphate (SDS), antibodies to only two protein antigens were found. These two proteins (A and B), while existing in a form apparently closely associated with peptidoglycan, could also be recovered from homogenates of whole organisms after sonication and from culture filtrates. Antigenic material was excreted throughout growth. SDS-polyacrylamide gradient gel electrophoresis showed A to have a molecular weight of 29 000, while B had a molecular weight of 190 000. Antigen B was purified to apparent homogeneity as judged by SDS-polyacrylamide gel electrophoresis and isoelectric focusing. All of six strains of serotype c examined produced antigen B. Strains of serotypes e and f also produce antigenically identical proteins and strains of serotypes d and g produce proteins which cross-reacted with antigen B. Antigen B was specifically precipitated by rabbit antiserum to human heart tissue.


PMID: 118231

DOI: 10.1099/00221287-114-1-109

Other references

Watkins, J.T., 1951: Beat knee. Practitioner 167(999): 257-264

Brockmeyer, N.H.; Kreuzfelder, E.; Guttmann, W.; Mertins, L.; Goos, M.; Ohnhaus, E.E., 1989: Cimetidine and the immuno-response in healthy volunteers. We compared the immunologic measurements from treatment of 12 healthy volunteers (six male, six female) with 800 and 1,600 mg cimetidine. In the first trial 800 mg cimetidine was administered daily to the volunteers over a period of 7 d; after an...

Ducoroy, P.; Lesourd, M.; Padros,; Tournefier, A., 1999: Natural and induced apoptosis during lymphocyte development in the axolotl. Lymphocytes apoptosis was characterized in a urodele amphibian, the axolotl, by morphology using electron microscopy and by flow cytometry after propidium iodide staining, as well as by biochemical criteria with the detection of DNA ladders after...

Ingledew, W.J.; Salerno, J.C.; Ohnishi, T., 1976: Thermodynamic resolution of cytochrome P450 and characterization of an iron-sulfur center in rat liver microsomal preparations. Archives of Biochemistry and Biophysics 174(1): 298-304

Leech R.M.; Rumsby M.G.; Thomson W.W.; Crosby W.; Wood P., 1972: Lipid changes during plastid differentiation in developing maize leaves. Forti, Giorgio, Mordhay Avron And Andrea Melandri (Ed ) Photosynthesis, Two Centuries After Its Discovery By Joseph Priestley Proceedings Of The 2nd International Congress on Photosynthesis Research, Vol 3 Stresa, Italy June 24-29, 1971 Xxiii+1055p Illus Dr W Junk N V Publishers: The Hague, The Netherlands 2479-2488

Hamid, S.; Rizvi, Q.K.; Ansari, S.; Farooqui, B.; Sultana, T.; Siddiqui, A.A., 1996: Needle stick injuries from hepatitis C positive patients Incidence and management. Gastroenterology 110(4 SUPPL ): A1203

Sweeney, M.P., 1965: Exhause Control Devices. Journal of the Air Pollution Control Association 15: 13-18

Piquery, L.; Davoine, C.; Huault, C.; Billard, J.P., 2000: Senescence of leaf sheaths of ryegrass stubble: changes in enzyme activities related to H2O2 metabolism. Changes in the levels of reactive oxygen species (O2(.)-, H2O2), and of activities of enzymes involved in their detoxification were investigated during senescence of leaf sheaths of ryegrass stubble. The accumulation of H2O2 in the medium leaf she...

Anonymous, 2006: Resolution of the plenary session of Research Council on Human Ecology and Environmental Hygiene of the Russian Academy of Medical Sciences and the Ministry of Health and Social Development of the Russian Federation on the Environment-Induced Human Damages: Guidelines, implication, and assessment prospects (Moscow, December 22-23, 2005). Gigiena i Sanitariya (5): 104-105

Kuck J.F.R.Jr, 1976: Effect of long wave uv light on the lens part 2 metabolic inhibitors synergistic with uv in vitro. Hockwin, O. Documenta Ophthalmologica Proceedings Series, Vol. 8. Progress Of Lens Biochemistry Research. Volume In Honour Of Prof. Dr. Med. J. Nordmann. Vii 316p. Illus. Dr. W. Junk Bv Publishers: The Hague, Netherlands. Isbn 90-6193-148-7. 261-265