+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

9,13-dicis-rhodopsin and its one-photon-one-double-bond isomerization

, : 9,13-dicis-rhodopsin and its one-photon-one-double-bond isomerization. Biochemistry 27(17): 6495-6499

Incubation of purified 9,13-dicis-retinal with cattle opsin in 2% digitonin at 20 degrees C produced two pigments, one unstable (lambda max 478 nm) and the other stable (lambda max 485 nm) in hydroxylamine. The two pigments exhibited different characteristics. HPLC analysis revealed that the chromophores of these pigments have respectively 9,13-dicis and 9-cis geometries. Under various conditions the amount of 9,13-dicis-rhodopsin formed never exceeded 30% of the total pigments. The addition of 9,13-dicis-retinal to the ROS suspension containing opsin produced 9-cis-rhodopsin in 97% yield. Irradiation of the 9,13-dicis-rhodopsin mainly produced 13-cis-retinal, while 9-cis-rhodopsin produced the all-trans isomer. These results demonstrated that the one-photon-one-double-bond isomerization process took place in 9,13-dicis-rhodopsin.


PMID: 2975508

Other references

Sato, S.; Furuya, N., 2017: Induction of PINK1/Parkin-Mediated Mitophagy. PINK1/Parkin mitophagy is a key mechanism to contribute mitochondrial quality control, and the defects are thought to be a cause of those Parkinson's disease onsets. Upon loss of mitochondrial membrane potential, PINK1 and Parkin are activate...

Karalazos, A.; Hatziminaoglou, J.; Sinapis, E., 1996: An effort for the improvement of the traditional sheep production system in a mountainous region of Greece. The effects of feeding supplements on milk yield and reproductive performance of native mountain breed ewes grazing in a mountain area of northern Greece were examined. In 2 experiments, 264 pregnant ewes 20 months of age (mean body weight 48.0 kg...

Savich, V.I.; Savich, L.V., 1994: Use of experimental designs with feedback to evaluate crop nutritional requirements and environmental pollution. Eurasian Soil Science 25(8): 92-102, 1993 ( )

Imuta, H.; Yasumura, S.; Fujita, M.; Arai, H.; Fukao, A., 1998: Homebound elderly in a Japanese community: related factors and change of mobility. The aim of this study was to identify the prevalence of homebound elderly (defined as people whose daily activities were limited to their home) and factors related to it among 300 community elderly residents aged 60 and over in Yamagata city. In 1...

Trieu, D.; Drosou, A.; Goldberg, L.H., 2015: Transposition pedicle flap of the posterior ear. Dermatologic Surgery 41(1): 155-157

Sato, A.; Choi, S.Won.; Hirai, M.; Yamayoshi, A.; Moriyama, R.; Yamano, T.; Takagi, M.; Kano, A.; Shimamoto, A.; Maruyama, A., 2007: Polymer brush-stabilized polyplex for a siRNA carrier with long circulatory half-life. Delivery systems of small interfering RNA (siRNA) are the key to siRNA therapeutic application. In this study, we prepared and evaluated a series of cationic comb-type copolymers (CCCs) possessing a polycationic backbone (less than 30 weight (wt)...

Oliva-Martin, M.Jose.; Sanchez-Abarca, L.Ignacio.; Rodhe, J.; Carrillo-Jimenez, A.; Vlachos, P.; Herrera, A.Jose.; Garcia-Quintanilla, A.; Caballero-Velazquez, T.; Perez-Simon, J.Antonio.; Joseph, B.; Venero, J.Luis., 2016: Caspase-8 inhibition represses initial human monocyte activation in septic shock model. NlmCategory="UNASSIGNED">In septic patients, the onset of septic shock occurs due to the over-activation of monocytes. We tested the therapeutic potential of directly targeting innate immune cell activation to limit the cytokine storm...

Sinibaldi, L.; Mancini, C.; Rivanera, D.; Goldoni, P., 1982: Antibody movement against BK and herpes viruses and cytomegalovirus in kidney transplants. Annali Dell'istituto Superiore di Sanita 18(3): 389-394

Soule, J.L.; Henry, M.L., 2007: Noninvasive methods to identify early vascular access dysfunction. A functional vascular access is of critical importance to the hemodialysis patient, the patient's healthcare providers, and the hemodialysis treatment center. A poorly functioning or thrombosed vascular access can lead to increased morbidity,...

Hansen, B.; Beck, C.; Townsley, R., 2003: Arthroscopic removal of a loose body osteophyte fragment after superior patellar dislocation with locked osteophytes. The authors report the case of a loose body from a fractured osteophyte after a superiorly dislocated patella with locked osteophytes. Few cases of superiorly dislocated patellae have been reported in the literature and no cases of osteophyte frac...