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A microculture technique for rat lymphocyte transformation

, : A microculture technique for rat lymphocyte transformation. Journal of Immunological Methods 30(1): 77-85

We report the development of an economical microculture technique suitable for measuring rat lymphocyte response to mitogens and in mixed lymphocyte reactions. The effects of varying culture conditions, i.e. source of serum, addition and concentration of 2-mercaptoethanol, mitogen concentrations, culture incubation times, absorption of serum, lymphocyte numbers and microtitre plate well shape are described.


PMID: 159926

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