geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A monoclonal anti-glycophorin A antibody recognizing the blood group M determinant: studies on the subspecificity


, : A monoclonal anti-glycophorin A antibody recognizing the blood group M determinant: studies on the subspecificity. Molecular Immunology 24(6): 605-613

A mouse monoclonal antibody (425/2B, IgM) was obtained which shows specificity for blood group M determinant of glycophorin A. The antibody is pH-dependent. At pH 6-7 it reacted strongly with blood group M antigen, but also cross-reacted distinctly with N antigen. At pH 8.3 the antibody showed moderately decreased reactivity with M antigen, but no interaction with N antigen was detectable by hemagglutination, immunoblotting, or microplate ELISA. The direct binding studies and inhibition of 425/B antibody by untreated or modified blood group M and N glycoproteins or tryptic glycopeptides showed that the binding to the antigens was not affected by acetylation of their amino groups, or removal of amino-terminal amino acid residue. Desialylation of the antigens decreased their reactivity with the antibody and this effect was distinctly stronger at pH 7 than 8.3. The antibody reacted strongly at pH 7 and 8.3 with glycophorin B of Henshaw phenotype, whereas its reactivity with normal glycophorin B was weak or undetectable at these pH values, respectively. The results obtained indicated that anti-M specificity of 425/2B antibody is related to the 5th amino acid residue of glycophorin A (anti-Mgly specificity) and that pH shift from 7 to 8.3 changes the fine specificity of the antibody. At pH 8.3 the reactivity of the antibody is more dependent on glycine residue (higher anti-M specificity) and less dependent on sialic acid residues in the antigen.

US$29.90

PMID: 2443838


Other references

Liu Sheng Fa; Yang Ting Bao, 2002: Haploporus mugilis sp n from Valamugil engeli in the Taiwan Straits, China. Haploporus mugilis sp. n. (Digenea: Haploporidae) was obtained from the intestine of kandas, Valamugil engeli (Bleeker) (Mugilidae, Perciformes) in the Taiwan Straits, China. It most closely resembles Haploporus spinosus Machida, 1996 from the int...

Glastris, P., 1985: Life and death in the emergency room. Washington Monthly 17(10): 20-31

Scott, E.Demond.; Gil, K.; King, B.Copeland.; Piatt, E., 2016: Clinical Outcomes in a Primary Care Practice Within a Center for Health Equity. A Center for Health Equity that included a primary care practice was built in a neighborhood affected by long-term disparities related to race and class in order to promote health equity within the population. Changes in blood pressure and HbA1c (...

Keulen, R.F.; Adam, J.J.; Fischer, M.H.; Kuipers, H.; Jolles, J., 2003: Distractor interference in selective reaching: dissociating distance and grouping effects. In the present experiment, the authors sought to differentiate between a distance and a grouping explanation for the symmetric versus asymmetric patterns of distractor interference in selective reaching. Participants (N = 16) pointed to a green ta...

Gooding, G.A.; Effeney, D.J., 1982: Static and real-time B-mode sonography of arterial occlusions. Static and real-time sonography can be used to confirm the clinical diagnosis of arterial occlusion. When occluded, both native arteries and grafts showed three sonographically detectable patterns. More often (18/23), the vessel or graft has a nor...

Mccall, R.G., 1953: Analysis of swimming pool turnover standard. United States Armed Forces Medical Journal 4(8): 1189-1193

Smith, M.; Geretti, A.Maria.; Osner, N.; Easterbrook, P.; Zuckerman, M., 2005: High levels of discordance between sequencing and serological subtyping in a predominantly non-B subtype HIV-1 infected cohort. Samples from 457 randomly selected HIV-1 infected patients attending King's College Hospital were analysed using a subtype specific enzyme immunoassay. All serotyped non-Bs that provided unambiguous sequence and for which sufficient sample wa...

Lindsey P.; Bassuk N., 1992: Redesigning the urban forest from the ground below a new approach to specifying adequate soil volumes for street trees. Arboricultural Journal 16(1): 25-39

Chowers, Y.; Cahalon, L.; Lahav, M.; Schor, H.; Tal, R.; Bar-Meir, S.; Levite, M., 2000: Somatostatin through its specific receptor inhibits spontaneous and TNF-alpha- and bacteria-induced IL-8 and IL-1 beta secretion from intestinal epithelial cells. Intestinal epithelial cells secrete proinflammatory cytokines and chemokines that are crucial in mucosal defense. However, this secretion must be tightly regulated, because uncontrolled secretion of proinflammatory mediators may lead to chronic in...

Weiss, S.A.; Smith, G.C.; Vaughn, J.L.; Dougherty, E.M.; Tompkins, G.J., 1982: Effect of aluminum chloride and zinc sulfate on Autographa california nuclear polyhedrosis virus (ACNPV) replication in cell culture. When IPL-SF-21AE III continuous insect cell line was grown and maintained in IPL-41 insect cell culture medium supplemented with 16 microM of AlCl3 or 0.24 microM of ZnSO4 . 7H2O, or both metallic salts, and then infected with Autographa californi...