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A new microsphere-based immunofluorescence assay using flow cytometry

, : A new microsphere-based immunofluorescence assay using flow cytometry. Journal of Immunological Methods 107(2): 225-230

The quantitative and qualitative capacities of flow cytometric analysis that have made it such a powerful tool in studies of cellular antigens have not previously been exploited when dealing with non-cellular antigens. A new immunofluorescence assay technique was developed, using an indirect staining procedure with monoclonal anti-kappa antibodies, to detect human free kappa light chains covalently bound to microspheres of a size suitable for flow cytometry. The strength of the fluorescent signal produced on the microspheres was related to the amount of antigen bound and the size of the beads. At the time of this work large microspheres (i.e., greater than 3 micron in diameter) suitable for this application were only available as suspensions of polysized beads. The fluorescent signal detected on labelled beads was optimized by selecting for analysis, on the basis of the forward angle laser scatter, only those beads of largest diameter. There are many potential applications for this technique - microspheres can be used for the presentation of virtually any antigen or antibody. The analytical benefits inherent in flow cytometry would be a significant advantage in the development of quantitative assays using this method.


PMID: 3126243

Other references

Xing, J.P.; Chen, B.Y.; Janes, H.W.; Gianfagna, T.J., 1997: Complete cDNA sequence of a tomato ADP/ATP translocator gene that is highly expressed in sink tissues. Plant Physiology (Rockville) 114(3 SUPPL ): 202

Deason T.R.; Ryals P.E.; O'kelley J.C.; Bullock K.W., 1979: Fine structure of mitosis and cleavage in friedmannia israelensis chlorophyceae chlorosarcinaceae. Observations on the ultrastructure of F. israelensis Chantanachat et Bold reveal the presence of a phycoplast and zoospores with cruciate rootlets. During mitosis, the nuclear envelope partially disintegrates and the basal bodies remain at the cel...

Dewdney J.M., 1979: Drugs as haptens. Turk, Jd D. Parker. (ed.). Drugs Amd Immune Responsiveness; Symposium, London, England, Apr. 17-18, . X 254p. University Park Press: Baltimore, Md., Usa. Illus. P161-168

Hynynen, K.; McDannold, N.; Vykhodtseva, N.; Jolesz, F.A., 2001: Noninvasive MR imaging-guided focal opening of the blood-brain barrier in rabbits. PURPOSE: To determine if focused ultrasound beams can be used to locally open the blood-brain barrier without damage to surrounding brain tissue and if magnetic resonance (MR) imaging can be used to monitor this procedure. MATERIALS AND METHODS: T...

Chen, J.J.; Tsu, C.A.; Gavin, J.M.; Milhollen, M.A.; Bruzzese, F.J.; Mallender, W.D.; Sintchak, M.D.; Bump, N.J.; Yang, X.; Ma, J.; Loke, H-Keng.; Xu, Q.; Li, P.; Bence, N.F.; Brownell, J.E.; Dick, L.R., 2012: Mechanistic studies of substrate-assisted inhibition of ubiquitin-activating enzyme by adenosine sulfamate analogues. Ubiquitin-activating enzyme (UAE or E1) activates ubiquitin via an adenylate intermediate and catalyzes its transfer to a ubiquitin-conjugating enzyme (E2). MLN4924 is an adenosine sulfamate analogue that was identified as a selective, mechanism-b...

Kashiwazaki, S.; Suzuki, S.; Okada, J., 1988: Side effects of immunomodulators and countermeasures. Ryumachi 28(6): 460-463

Tranter, 2000: Reflecting on the modern industrial life-science environment. 'The barriers between 'silos' in pharma R&D workflows are slowly eroding and this is driving a wider exchange of information across the various R&D disciplines'

Azuma A.; Okuno Y., 1987: Flight of a samara alsomitra macrocarpa. The steady gliding flight of samples of Alsomitra macrocarpa samara was filmed and analysed. By using the observed data, the flight performance of the samara was made clear. The lift-to-drag ratio or the gliding ratio was about 3 .apprx. 4 and the...

Imai, Y., 1978: Fluoridation in Japan: Interpretation of the optimum concentration in Japan. Shikai Tenbo 51(6): 1217-1225

Ruiter, M.; Meyler, L., 1960: Skin changes after the therapeutic use of high doses of nicotinic acid. Nederlands Tijdschrift Voor Geneeskunde 104: 1114-1116