geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A rapid microassay for measuring the luminol-dependent chemiluminescent response in canine whole blood


, : A rapid microassay for measuring the luminol-dependent chemiluminescent response in canine whole blood. Veterinary Immunology and Immunopathology 29(3-4): 285-293

A microassay for the luminol-dependent chemiluminescence (CL) response in canine whole blood was developed to measure indirectly the oxidative metabolism of peripheral blood leukocytes. Fifty microliters of blood were mixed with 705 microliters of Hank's balanced salt solution containing 25 mM Hepes and 1.3 x 10(-4) M luminol. This mixture was allowed to equilibrate for 5 min after which 60 microliters of latex beads (0.801 microns diameter) were added as a stimulant, and the CL response was monitored continuously for 5 min at 37 degrees C using a luminometer. The whole blood CL response was significantly correlated (r = 0.784, P less than 0.01, n = 14) with the number of neutrophils in the peripheral blood. Further, the whole blood CL response was abolished by the depletion of neutrophils after passing the blood through an adherence column and by the addition of sodium azide. The relative chemiluminescent light unit (RCLU) was a reliable marker for comparing each peak value in different samples. The coefficient of variation (CV) of repetitive samples was 9.87%, and the CV of 14 normal dogs was 15.7%. This method is useful and applicable for screening the CL response in canine whole blood.

US$29.90

PMID: 1949590


Other references

Ruijs, A.; Zimmermann, A.; Van-Den-Berg, M., 2008: Demand and distributional effects of water pricing policies. Worldwide, water scarcity threatens delivery of water to urban centers. water pricing is often recommended to reduce demand. In this paper, demand and distributional effects of water pricing policies are examined in a block pricing model that is...

Melamed, R.; Greenberg, D.; Landau, D.; Khvatskin, S.; Shany, E.; Dagan, R., 2002: Neonatal nosocomial pneumococcal infections acquired by patient-to-patient transmission. A case of neonatal nosocomial pneumococcal sepsis acquired by patient-to-patient transmission and confirmed by phenotypic and genotypic typing is documented. To the best of our knowledge this is the first documented case of neonatal nosocomial per...

Somogyvari, K.S.gi, L.Z.oldos, S.P.rkany, M., 1981: Experiences in the new formation of hyalin cartilage on the joint surface. Schweizer Archiv fur Tierheilkunde 123(7): 345-353

Leu, H.J., 1990: Chronic venous insufficiency today (a status determination). Primary as well as secondary cvi (due to deficiency of the deep venous reflux) is a disease that necessitates a thorough therapy planning and a life-long medical care. Neither operation nor sclerotherapy alone can cure these patients. Active measu...

Eggers, C.; Klapal, M., 1997: Diagnostic and follow-up typological characteristics of early schizophrenia. Schizophrenic psychoses with early onset (< or = 10) are very rare; they occur in approx. 0.5-1% of the total number of patients. Long-term research with sufficiently long observation periods may be able to answer the question whether there is...

Kruczalowa, M.; Weiss, A., 1968: Diagnosis and treatment of allergic diseases by means of allergens and vaccines of domestic production. Polski Tygodnik Lekarski 23(11): 405-406

Lovendahl, P.; Liboriussen, T.; Jensen, J.; Vestergaard, M.; Sejrsen, K., 1994: Physiological predictors in calves of dairy breeds: Part 1. Genetic parameters of basal and induced growth hormone secretion. Genetic variation in plasma growth hormone (GH) concentration before and after GRF (growth hormone releasing factor) stimulation was studied in young bulls (N = 284) and heifers (N = 272), the progeny of 53 sires of four dairy and dual-purpose bre...

Schulz, H.D.; Kluge, H.H.; Korda, W.; Kube, B., 1977: Treatment of schizophrenic psychoses with Lyorodin. Forty-four patients suffering from acute and chronic schizophrenic psychoses were used to obtain, by using Lorr's scale (IMPS) and taking the changes in disease state observed within three months as a base, suggestions or pointers as to the p...

Paletta B.; Binder M.; Paschke E., 1975: The time function of gluco cortico steroids in rat plasma under hypoxia stress. Hormone & Metabolic Research 7(3): 269-270

Fowlkes, John, L., 1997: Insulinlike growth factor-binding protein proteolysis: An emerging paradigm in insulinlike growth factor physiology. In biologic fluids, insulinlike growth factors (IGF-I and IGF-II) are bound to high-affinity insulinlike growth factor binding proteins (IGFBPs), of which seven have now been identified (IGFBPs 1-7). In a variety of biologic fluids, several IGFBPs...