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A thermodynamic study of 5-[3H]hydroxytryptamine binding to human cortex membranes


, : A thermodynamic study of 5-[3H]hydroxytryptamine binding to human cortex membranes. Journal of Neurochemistry 49(5): 1480-1483

Kinetic and equilibrium measurements of [3H]-serotonin (5-hydroxytryptamine) binding to human frontal cortex membranes have been made between 4 and 30 degrees C. The effects of spiperone and ascorbate on binding have also been determined. Under the conditions used, binding was saturable and reversible. Affinity constants derived from kinetic and equilibrium data were comparable. Serotonin binding to several sites had substantial enthalpic as well as entropic components.

US$29.90

PMID: 3668534


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