+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Acrylamide-induced increases in deposition of axonally transported glycoproteins in rat sciatic nerve

, : Acrylamide-induced increases in deposition of axonally transported glycoproteins in rat sciatic nerve. Journal of Neurochemistry 52(4): 1240-1247

The axonal transport of proteins, glycoproteins, and gangliosides in sensory neurons of the sciatic nerve was examined in adult rats exposed to acrylamide via intraperitoneal injection (40 mg/kg of body weight/day for nine consecutive days). The L5 dorsal root ganglion was injected with either [35S]methionine to label proteins or [3H]glucosamine to label, more specifically, glycoproteins and gangliosides. At times ranging from 2 to 6 h later, the sciatic nerve and injected ganglion were excised and radioactivity in consecutive 5-mm segments determined. In both control and acrylamide-treated animals, outflow profiles of [35S]methionine-labeled proteins showed a well defined crest which moved down the nerve at a rate of approximately 340 mm/day. Similar outflow profiles and transport rates were seen for [3H]glucosamine-labeled glycoproteins in control animals. However, in animals treated with acrylamide, the crest of transported labeled glycoprotein was severely attenuated as it moved down the nerve. This finding suggests that in acrylamide-treated animals, axonally transported glycoproteins were preferentially transferred (unloaded or exchanged against unlabeled molecules) from the transport vector to stationary axonal structures. We also examined the clearance of axonally transported glycoproteins distal to a ligature on the nerve. The observed impairment of clearance in acrylamide-treated animals relative to controls is supportive of the above hypothesis. Acrylamide may directly affect the mechanism by which axonally transported material is unloaded from the transport vector. Alternatively, the increased rate of unloading might reflect an acrylamide-induced increase in the demand for axonally transported material.


PMID: 2926398

Other references

Maresca, A.; Thayer, R.E.; Gosselin, P.; Singer, M.F., 1987: Genomic organization of low copy number sequences that are associated with deca-satellite DNA in the monkey genome. A previously described segment of African green monkey DNA (cloned in phage lambda MkA) contains deca-satellite linked to DNA sequences that are estimated to occur once per genome. Sequences homologous to the low copy number sequences in lambda Mk...

Wakabayashi, K., 1964: Enzymatic Hydrolysis Of L-Prolyl-Beta-Naphthylamide And A Colorimetric Assay Method For Prolylpeptide Hydrolase Activity. Journal of Biochemistry 55: 244-253

Goudreault, Serge., 1993: Capture d'un Boloria selene atrocostalis (Huard) (Lepidoptera: Nymphalidae) melanique pres de Ste-Anne-de-la-Pocatiere. Fabreries. Septembre; 183: 91-92

Ermer P.M.; Miller P.S.; Lewis A.J.; Giesemann M.A., 1992: Spray dried porcine plasma in diets for lactating sows. Journal of Animal Science 70(SUPPL 1): 72

Bazhenov, L.G.; Iskhakova, K.I., 1983: Methods of isolation and identification of Bacteroides (review of the literature). Laboratornoe Delo: 3-6

Manríquez, J.; Berroeta-Mauriziano, D.; Andino-Navarrete, R.; Vera-Kellet, Cán., 2015: Scleromyxedema with neurological symptoms: successful treatment with immunoglobulins. Medicina Clinica 144(1): 47-48

Anonymous, 1889: Gray's Torsional Testing-Machine. Science 13(319): 194-194

Eto, S., 1989: Changes in gastric mucosal blood flow after selective proximal vagotomy; an experimental study in rats. The effect of selective proximal vagotomy (SPV) on gastric mucosal blood flow (MBF) was studied in rats using hydrogen gas clearance technique. The following results were obtained. 1) Resting MBF of the corpus decreased significantly from 106.3 +/...

Ploshchanskyĭ, V.V., 1992: Incarcerated umbilical hernia with necrosis of the ileum and right colon. Klinicheskaia Khirurgiia: 64-64

D'Souza, P., 2017: Notable licensing deals in the biopharma industry in the second quarter of 2017. During the second quarter of 2017, Cortellis Competitive Intelligence added 967 new licensing deals (excluding mergers and acquisition deals) as part of its ongoing coverage of pharmaceutical licensing activity. This meant an 8% decrease on the pr...