+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase

, : Addition of mononucleotides to oligoribonucleotide acceptors with T4 RNA ligase. Proceedings of the National Academy of Sciences of the United States of America 75(3): 1270-1273

RNA ligase induced by bacteriophage T4 catalyzed the addition of nucleoside 5',3'-diphosphates to oligoribonucleotide acceptors in the presence of ATP. The reactions proceeded in equimolar concentrations of donors and acceptors. Several oligonucleotides of defined sequence were synthesized by this method in yields of 28-68%. The enzyme required the phosphate ester at the 3' position of the donor molecule, nucleoside 5',2'-diphosphates being unable to serve as donors. Thymidine 5',3'-diphosphate was active as a donor for the enzyme. The ligation product, (Ap)2ApCp, was also obtained from the reaction of (Ap)2A and 5'-adenylylated cytidine 5',3'-diphosphate (A5'pp5'Cp) with RNA ligase in the absence of ATP. These results show that the minimal substrate for RNA ligase is a nucleoside 5',3'-diphosphate.


PMID: 274717

Other references

Xu, C.; Pranovich, A.; Vähäsalo, L.; Hemming, J.; Holmbom, B.; Schols, H.A.; Willför, S., 2008: Kinetics of acid hydrolysis of water-soluble spruce O-acetyl galactoglucomannans. Water-soluble O-acetyl galactoglucomannan (GGM) is a softwood-derived polysaccharide, which can be extracted on an industrial scale from wood or mechanical pulping waters and now is available in kilogram scale for research and development of valu...

Alperin, P.M.; Agranenko, V.A.; Rodina, R.I., 1965: Anemia In Patients With Acute Renal Insufficiency. Forty patients with acute renal insufficiency were examined. Hyporegenerative normochromic or hypochromic (more rarely hyperchromic) anemia of macrocytic type was revealed in all the patients. The chief cause of anemia in acute renal insufficiency...

Marsac, J.; Pujet, J.C., 1983: Respiratory handicap. Recognition, evaluation and social benefits. The medico-social aspects of respiratory handicap pose some perplexing problems, notably in their recognition, rigorous evaluation and in the granting of social security benefits. The clinical and respiratory function data should be standardised a...

Gel man M.L., 1970: Magmatism; General characterization. Northeast USSR.

Greenwell, Br, 1978: Chemical control of root weevils. Combined proceedings8(28): 83-85

Kawaguchi, K.; Faulk, K.; Purcell, R.H.; Emerson, S.U., 2011: Reproduction in vitro of a quasispecies from a hepatitis C virus-infected patient and determination of factors that influence selection of a dominant species. Hepatitis C virus infections proceed to chronicity in the majority of cases. In patients, hepatitis C viruses exist as a dynamic and complex quasispecies. The dominant species at any one time arises in response to host immune pressure and other, i...

Cacciari, E.; Tassoni, P.; Cicognani, A.; Dallera, L.; Miano, A., 1969: Relation between glycemia, blood insulin and blood cortisol in obese children during various tests of functional exploration of the endocrine pancreas. La Clinica Pediatrica 51(11): 635-649

S.L.uis, E.K.; Wijdicks, E.F.; Li, H., 1998: Predicting neurologic deterioration in patients with cerebellar hematomas. Background: Patients with cerebellar hematomas may appear stable but may worsen suddenly. Whether certain clinical or CT scan findings predict worsening is not known. Methods: We reviewed clinical and neuroimaging data in 72 patients with cerebell...

Weber, M., 1895: Fische von Ambon, Java, Thursday Island, dem Burnett-Fluss und von der Sudkuste von New-Guiuea. Semon's Zoologische Forschungsreisen, V 259-276

Potthast, K., 1977: Determination of phenols in smoked meat products. By means of steam distillation at high boiling temperature ( C) the phenolic compounds of smoke were isolated from smoked meat products. Eugenol, siringaldehyde acetosyringgone and acetovanillone are predominant in hot smoked meat p...