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Antibody-nucleic acid interactions. Antibodies to psoralen-modified RNA as probes of RNA structure

, : Antibody-nucleic acid interactions. Antibodies to psoralen-modified RNA as probes of RNA structure. Nucleic Acids Research 15(21): 9057-9073

Antisera elicited by immunization of rabbits with 4'-aminomethyl-trioxsalen (AMT)-modified poly(A,U) complexed with methylated bovine serum albumin was characterized in competition radioimmunoassays (RIA) and enzyme-linked immunosorbent assays (ELISA). AMT-poly(A,U) was over 10,000-fold more reactive than unmodified poly(A,U) or AMT alone. The antiserum cross-reacted to varying extents with AMT-modified-RNA's and -DNA's. The presence of AMT-uridine usually assured strong reactivity. The amino group of AMT contributed to antibody binding to a small degree. Binding was not significantly affected by high ionic strength, suggesting that binding does not involve ion pair formation. Murine encephalomyocarditis virus replicative intermediates, as well as cellular RNA and DNA were modified by psoralen in intact cells, suggesting that EMCV RNA and cellular RNA's in intact cells possess detectable stretches of base pairs. The antibodies described here will be useful in studying the secondary and tertiary structure of RNA's in vitro and in intact cells.


PMID: 2446261

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Curtiss, F.R.; Fairman, K.A., 2010: No TREATment with darbepoetin dosed to hemoglobin 13 grams per deciliter in type 2 diabetes with pre-dialysis chronic kidney disease--safety warnings for erythropoiesis-stimulating agents. Journal of Managed Care Pharmacy 15(9): 759-765

Hermes, I.H.; Khattab, M.S.; Ahamed, E.G., 1988: Studies on the nutritional value of students' restaurant food waste and its effect on the performance of growing chickens. 2. Productivity performance. For 7 weeks, 400 Lohmann and for 17 weeks, 400 Fayoumi chickens, 2 weeks old, were kept in battery or floor systems and given diets containing 0, 5, 10 or 15% dry student restaurant food waste (DSRFW). Average liveweight for Lohmann chickens was 1...

Klebesadel L.J., 1969: Winter survival and spring forage yield of winter rye m cultivars in sub arctic alaska as influenced by date of planting. Agronomy Journal 61(5): 708-712

Fishman, A.P., 1972: Pulmonary edema. The water-exchanging function of the lung. Circulation 46(2): 390-408

Jamieson, G.R.; Reid, E.H., 1976: Gas liquid chromatography characteristics of some long chain acetylenic methyl esters. Methyl esters from the lipids of Fontinalis antipyretica were separated by preparative TLC on silica gel and examined with GLC. Retention data are given for C18 and C20 polyolefinic acids. Type I and II separation factors are also given. For acety...

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Gordon, H.Mcl., 1952: Worm parasites in horses. As practically every horse is infected with helminths and the environment is continuously being contaminated, control measures must be continuous and aim at a reduction to low levels. Better feeding, good management, dean pastures and stables are...

Anderson A.T., 1968: The oxygen fugacity of alkaline basalt and related magmas, Tristan da Cunha. Intergrown phenocrystic titaniferous magnetite-ferrian ilmenite, reduction of ferrian ilmenite by liquid rather than by oxidation of titaniferous magnetite, oxygen fugacity-temperature relationships as criteria of mode of origin.

Duffy D.C., 1986: Foraging at patches interactions between common sterna hirundo and roseate terns sterna dougalli. In foraging groups in the Long Island Sound area of the eastern United States, Roseate Terns Sterna dougalli plunged for longer periods and from greater heights than did Common Terns Sterna hirundo. Roseate Terns were rarer than expected in dense...

Egozcue J., 1986: Aneuploidy maternal age and sexual immaturity. American Journal Of Human Geneticssuppl: A112