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Application of immunochemical determination methods in foreign protein analysis. 1. Determination and identification of faba bean protein isolate by means of the rocket technic on cellulose acetate film and countercurrent immunoelectrophoresis


, : Application of immunochemical determination methods in foreign protein analysis. 1. Determination and identification of faba bean protein isolate by means of the rocket technic on cellulose acetate film and countercurrent immunoelectrophoresis. Die Nahrung 29(7): 685-691

The present work deals with the applicability of the Rocket technique on cellulose acetate film and of the countercurrent immunoelectrophoresis for identification or determination of faba bean protein in model foods. It was found that both the methods are suitable for a rapid and uncomplicated determination or identification of faba bean protein in not heat-denaturized food. Cross reactivities occur with other leguminous proteins existing in the samples, effects of which add with those of the faba bean protein additive if the Rocket technique is used. With determination of faba protein by means of the Rocket-technique on cellulose acetate film coefficients of variation of about 20% are obtained.

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PMID: 4047135


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