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Application of ion-exchange and lipophilic-gel chromatography to the purification and group fractionation of steroidal spirolactones, isolated from biological fluids


, : Application of ion-exchange and lipophilic-gel chromatography to the purification and group fractionation of steroidal spirolactones, isolated from biological fluids. Journal of Chromatography 153(1): 63-75

Chromatography of steroidal spirolactones on DEAE-Sephadex A-25 under selected pH conditions allowed efficient separation of these compounds from other steroids and many of the endogenous components of human urine. The spirolactones were recovered in high yield, mostly over 90%. Lipophilic-gel chromatography provided a useful method for group fractionation of mixtures of these spirolactones with high recoveries (generally over 90%), unaffected by the presence of endogenous material from normal human urine.

US$29.90

PMID: 659562


Other references

David, H.A.; Paulson, A.S., 1965: The performance of several tests for outliers. Biometrika 52(3): 429-436

Senkbeil, J.C.; Brommer, D.M.; Comstock, I.J.; Loyd, T., 2012: Hydrometeorological application of an extratropical cyclone classification scheme in the southern United States. Extratropical cyclones (ETCs) in the southern United States are often overlooked when compared with tropical cyclones in the region and ETCs in the northern United States. Although southern ETCs are significant weather events, there is currently n...

Reddy, D.V.S.; Subrahmanyam, K.V., 2014: The buffalo; need for intensive studies on the natural history, morphology and physiology of the buffalo. Indian Veterinary Journal 27(1): 18-22

Song, H-Can.; Li, W-Ming.; Liu, G-Rong.; Xu, Z-Le., 2003: Study on the optical properties of 4,4'-bis-(2-(substituted-styryl))biphenyl and 1,4-bis-(2-(substituted-styryl))benzene. The maximum absorption wavelengths (lambda(a-max)), absorption coefficient (epsilon), maximum emission wavelengths (lambda(e-max)), fluorescent quantum yields (phi(f)), and second-order nonlinear polarizations (beta(xxx)) of seventeen 4,4'-bi...

Sambhandharaksa S.; Srieium T., 1991: Slope analysis of Pasak River bank. Proceedings of the Asian Regional Conference on Soil Mechanics and Foundation Engineering 9, Vol

Gurbuchev, I., 1977: Increase of phosphorus fertilizer efficiency in Bulgaria. II. significance of phosphate forms in mineral fertilizers (Increase of the effectiveness of raw phosphorites). Pochvoznanie i agrokhimiia: 2 (3) 53-60

White, K.; Berbaum, K.; Smith, W.L., 1994: The role of previous radiographs and reports in the interpretation of current radiographs. RATIONALE AND OBJECTIVES. The authors studied radiologists' patterns of using old reports and previous radiographs in the interpretation of current radiographic studies. Supplying information from previous studies is considered necessary for...

Cabral, A.L.; Park, R.F., 2016: Genetic analysis of seedling resistance to crown rust in five diploid oat (Avena strigosa) accessions. Crown rust, caused by Puccinia coronata Corda f. sp. avenae Eriks., is a serious menace in oats, for which resistance is an effective means of control. Wild diploid oat accessions are a source of novel resistances that first need to be characteris...

Wei E., 1978: Interaction of opiates with agents which cause withdrawal like syndromes. Adler, Martin W , Luciano Manara And Rosario Samanin (Ed ) Monographs Of The Mario Negri Institute For Pharmacological Research Factors Affecting The Action Of Narcotics Proceedings Of A Meeting Milan, Italy Xxii+774p Illus Raven Press: New York, N Y , Usa Isbn 0-89004-272-1 147-157

Mann T., 1973: Energy requirements of spermatozoa and the cervical environment. Blandau, Richard J And Kamran Moghissi (Ed ) The Biology Of The Cervix Symposium Xi+450p Illus University Of Chicago Press: Chicago, Ill , U S A ; London, England 329-338