geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Application of ion-pair high performance liquid chromatography for analysis of hyoscyamine and scopolamine in solanaceous crude drugs


, : Application of ion-pair high performance liquid chromatography for analysis of hyoscyamine and scopolamine in solanaceous crude drugs. Chemical & Pharmaceutical Bulletin 37(9): 2456-2458

A new ion-pair high-performance liquid chromatographic method for the analysis of hyoscyamine and scopolamine in various kinds of crude drugs derived from solanaceous plants was evaluated using sodium dodecyl sulfate as a counter ion. A reversed-phase chromatographic system consisting of a chemically bonded ODS silica gel column with phosphate buffer (pH 2.5)-acetonitrile (65:35) containing 17.5 mM sodium dodecyl sulfate as the mobile phase was used. Hyoscyamine and scopolamine in crude drugs derived from Scopolia, Atropa, Hyoscyamus and Datura species were separated from other compounds in the crude drugs and determined within 20 min after direct injection of the solution extracted with the mobile phase. The results for various kinds of samples are presented.

US$29.90

PMID: 2605691


Other references

Castiglia, C.L., 1988: A comparison of methods for analysis of mRNA in hematopoietic cells: conventional and clonal northern analysis and in situ hybridization. Three methods for the detection of mRNA in hematopoietic cells are addressed, and examples using these techniques to examine human leukemic cells' genetic response to chemical and biological response modifiers shown. The limitations and speci...

High, K.A., 2001: Gene therapy: a 2001 perspective. In the past year, three clinical trials of gene therapy for haemophilia have been initiated. Years of preclinical studies have culminated in translation of research findings into the clinical arena. It is too early to predict which, if any, of the...

Smielak-Szpunar, Z., 1966: Aneurysm and rupture of the left ventricle as a complication of myocardial infarction. Polski Tygodnik Lekarski 21(47): 1809-1812

Bolognesi, M.Maria.; Manzoni, M.; Scalia, C.Rossana.; Zannella, S.; Bosisio, F.Maria.; Faretta, M.; Cattoretti, G., 2017: Multiplex Staining by Sequential Immunostaining and Antibody Removal on Routine Tissue Sections. Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in situ, has raised considerable interest. The methods proposed include the use of labeled primary antibodies, spectral separation of fluorochromes, bleaching...

Reymond, J.C., 1950: What one should know about chloromycetin. Journal des Praticiens; Revue Generale de Clinique et de Therapeutique 64(21): 257-258

Espinoza, W.; Azevedo, J.A. de; Reis, A.E.G.D., 1980: Influence of soil cover on the field moisture regimes of two cerrado oxisols: LVE and LVA. During the 1978/1979 agricultural season, a study was carried out in two "cerrado" oxisols: LVE (Typic Haplustox) and LVA (Haplustox-Acrustox) having the following covers: bare soil, rice (Oryza sativa L., cv. IAC-25), corn (Zea mays L.,...

Ross K.G.; Matthews R.W., 1982: 2 polygynous over wintered vespula squamosa colonies from the southeastern usa hymenoptera vespidae. Two overwintered colonies of V. squamosa (Drury) are described and compared to conspecific annual colonies from the same region. Volumes occupied by the overwintered nests were 35 and 60 l with total cell counts of 65,000 and 95,000, respectively....

Gabrielyan E.S.; Ordyan M.B.; Tatebosyan A.T.; Madakyan V.N.; Matevosyan R.Sh, 1984: Study of the physiological activity of some derivatives of porphyrins. The physiological activity of some porphyrin derivatives and their metal-complexes were studied on systemic arterial pressure, cerebral blood circulation, microcirculation and oxigenic strain in the brain. Their antispastic effects were studied as...

Freitas-Junior, R.; Freitas, N.Maya.Aires.; Curado, M.Paula.; Martins, E.; Moreira, M.Amaral.Rebouças.; e Silva, C.Maciel.Bandeira., 2008: Variations in breast cancer incidence per decade of life (Goiânia, GO, Brazil): 16-year analysis. To analyze the variation in breast cancer incidence, with stratification into ten-year age groups, 17 years after the nuclear accident in Goiânia. Between 1988 and 2003, 3312 new cases were recorded. The crude and standardized incidence rates wer...

Krygier, Jt, 1970: Changes in water yield and peak flows from land management. Manage young growth Douglas fir and west hemlock: 54-58